التفاصيل البيبلوغرافية
| العنوان: |
LncRNA-H19 activates CDC42/PAK1 pathway to promote cell proliferation, migration and invasion by targeting miR-15b in hepatocellular carcinoma. |
| المؤلفون: |
Zhou, Yong1 (AUTHOR), Fan, Ren-Gen1 (AUTHOR), Qin, Cheng-Lin1 (AUTHOR), Jia, Jing2 (AUTHOR), Wu, Xu-Dong1,3 (AUTHOR) hnjsycwxd@163.com, Zha, Wen-Zhang1 (AUTHOR) zhawenzhang@sina.cn |
| المصدر: |
Genomics. Dec2019, Vol. 111 Issue 6, p1862-1872. 11p. |
| مصطلحات موضوعية: |
*HEPATOCELLULAR carcinoma, *CELL proliferation, *WESTERN immunoblotting, *FLOW cytometry, *CELL migration inhibition, *CAUSES of death |
| مستخلص: |
Hepatocellular carcinoma (HCC) is one of the main causes of cancer-related death. This study aims to explore the role and underlying mechanism of H19 in HCC. qRT-PCR detected miR-15b-5p and H19 expression, as well as the mRNA level of EMT-associated genes. Western blotting detected protein level of EMT-associated genes. Immunohistochemistry (IHC) examined CDC42 in HCC tissues. Dual luciferase reporter assay verified the regulatory mechanism among H19, miR-15b and CDC42. Colony formation, wound healing assay, transwell, flow cytometry measured proliferation, migration, invasion and apoptosis, respectively. H19 and CDC42 were up-regulated while miR-15b was down-regulated in HCC cells and tissues. miR-15b interacted with H19 and CDC42 3′-UTR. H19 knockdown inhibited proliferation, migration and invasion, and increased apoptosis, which was rescued by miR-15b inhibitor. H19 knockdown suppressed CDC42/PAK1 pathway and EMT progress. H19 knockdown inhibited proliferation, migration and invasion, and promoted apoptosis of HCC cells via targeting miR-15b/CDC42/PAK1 axis. • H19 and CDC42 are up-regulated and miR-15b is down-regulated in HCC. • H19 positively regulates CDC42 via sponging miR-15b. • H19 knockdown suppresses proliferation and promotes apoptosis by up-regulating miR-15b. • H19 knockdown and miR-15b overexpression repress invasion and migration by inhibiting EMT. • H19 knockdown inhibits CDC42/PAK1 pathway by targeting miR-15b. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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