التفاصيل البيبلوغرافية
| العنوان: |
Antigenic regions of human chromogranin A and their topographic relationships with structural/functional domains. |
| المؤلفون: |
Corti, Angelo1, Longhi, Renato2, Gasparri, Anna1, Fuxiang Chen1, Pelagi, Micaela1, Siccardi, Antonio G.1,3 |
| المصدر: |
European Journal of Biochemistry. 1/15/96, Vol. 235 Issue 1/2, p275-280. 6p. |
| مصطلحات موضوعية: |
*CHROMOGRANINS, *ANTIGENS, *TUMOR markers, *RADIOIMMUNOIMAGING, *MONOCLONAL antibodies, *NEUROENDOCRINE cells |
| مستخلص: |
Chromogranin A is a protein contained in the secretory granules of many neuroendocrine cells. The linear antigenic sites of human chromogranin A were studied by examining the cross-reaction of polyclonal and monoclonal anti-chromogranin A antibodies with native chromogranin A and with synthetic peptides encompassing most of the chromogranin A sequence. Chromogranin A residues 1-20, 47-67. 107-158, 254-297, 331-375, and 395-419 were found to be poorly or not antigenic, while residues 25-46, 163-210, 231-253, 298-314 and 68-106, 222-230, 315-330, 376-394 were found to contain weak and strong antigenic sites, respectively. Residues 68-70 (GAK) and 81-90 (GFEDELSEVL) were strongly recognized by two mouse mAbs (B4E11 and A11, respectively). Since mAb A11 has been previously used for immunohistochemical analysis of chromogranin-A-producing tissues from different species and for in vivo imaging of chromogranin-A-positive endocrine tumors, these results imply that at least part of the 81-90 region is surface-exposed in cryostat tissue sections as well as in vivo. The results may help in selecting new antibodies with improved affinity and immunogenicity for in vivo targeting of chromogranin-A-producing tumors. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder