التفاصيل البيبلوغرافية
| العنوان: |
Structural and functional study of ChuY from Escherichia coli strain CFT073. |
| المؤلفون: |
Kim, Hun1, Chaurasia, Akhilesh Kumar1, Kim, Truc1, Choi, Jongkeun2, Ha, Sung Chul3, Kim, Doyoun1, Kim, Kyeong Kyu1 kyeongkyu@skku.edu |
| المصدر: |
Biochemical & Biophysical Research Communications. Jan2017, Vol. 482 Issue 4, p1176-1182. 7p. |
| مصطلحات موضوعية: |
*ESCHERICHIA coli, *FLAVIN mononucleotide, *BILIVERDIN, *URINARY tract infections, *PORPHYRINS |
| مستخلص: |
The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 Å resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. A chuY deletion-insertion strain showed reduced survival potential compared to wild-type and complemented strains in mammalian cells. Current results suggest ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of E . coli CFT073. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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