التفاصيل البيبلوغرافية
| العنوان: |
Glucagon-like peptide-1 prevented abdominal aortic aneurysm development in rats. |
| المؤلفون: |
Yu, Jie1, Morimoto, Keisuke1, Bao, Wulan1, Yu, Zhenhai1, Okita, Yutaka1, Okada, Kenji2 yutamo@aol.com |
| المصدر: |
Surgery Today. Sep2016, Vol. 46 Issue 9, p1099-1107. 9p. |
| مصطلحات موضوعية: |
*GLUCAGON-like peptide-1 receptor, *AORTIC aneurysms, *PHARMACODYNAMICS, *REACTIVE oxygen species, *CELLULAR signal transduction, *IMMUNOSTAINING, *LABORATORY rats |
| مستخلص: |
Purpose: To demonstrate the protective effect of glucagon-like peptide 1 (GLP-1) signaling on the cardiovascular system, we conducted this study to show that the GLP-1 receptor analog (lixisenatide) could inhibit abdominal aortic aneurysm (AAA) development in rats. Methods: Lixisenatide was injected subcutaneously 7 days after aneurysm preparation. We evaluated reactive oxygen species (ROS) expression by dihydroethidium staining and 8-hydroxydeoxyguanosine (8-OHdG; the oxidation product of DNA) by immunohistochemical staining. We also analyzed the effect of GLP-1 signaling on the inflammatory response. Histopathological examination was done on day 28, and the AAA dilatation ratio was calculated. Results: On day 14, ROS expression and 8-OHdG-positive cells in the aneurysm walls were seen to have been significantly decreased by lixisenatide treatment. Western blot analysis showed decreased ERK expression. There was significantly reduced tumor necrosis factor-α mRNA expression in the aneurysm walls and CD68-positive cell infiltration in the aneurysm walls. On day 28, it was evident that the lixisenatide had dramatically reduced aneurysm development in the rats. Conclusion: GLP-1 elevation inhibits AAA development in rats through its anti-oxidant and anti-inflammatory effects. Thus, GLP-1 could be a potent pharmacological target for AAA treatment. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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