التفاصيل البيبلوغرافية
| العنوان: |
Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids. |
| المؤلفون: |
Kato, Takuma1, Yamashita, Hiroko2,3, Misawa, Takashi2, Nishida, Koyo1, Kurihara, Masaaki2,3, Tanaka, Masakazu1, Demizu, Yosuke2 demizu@nihs.go.jp, Oba, Makoto1 moba@nagasaki-u.ac.jp |
| المصدر: |
Bioorganic & Medicinal Chemistry. Jun2016, Vol. 24 Issue 12, p2681-2687. 7p. |
| مصطلحات موضوعية: |
*PEPTIDES, *AMINO acids, *GENE delivery techniques, *ENDOSOMES, *LYSOSOMES |
| مستخلص: |
Cell-penetrating peptides (CPPs) have been developed as drug, protein, and gene delivery tools. In the present study, arginine (Arg)-rich CPPs containing unnatural amino acids were designed to deliver plasmid DNA (pDNA). The transfection ability of one of the Arg-rich CPPs examined here was more effective than that of the Arg nonapeptide, which is the most frequently used CPP. The transfection efficiencies of Arg-rich CPPs increased with longer post-incubation times and were significantly higher at 48-h and 72-h post-incubation than that of the commercially available transfection reagent TurboFect. These Arg-rich CPPs were complexed with pDNA for a long time in cells and effectively escaped from the late endosomes/lysosomes into the cytoplasm. These results will be helpful for designing novel CPPs for pDNA delivery. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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