التفاصيل البيبلوغرافية
| العنوان: |
CTCF Binding Polarity Determines Chromatin Looping. |
| المؤلفون: |
de Wit, Elzo1, Vos, Erica S.M.1, Holwerda, Sjoerd J.B.1, Valdes-Quezada, Christian1, Verstegen, Marjon J.A.M.1, Teunissen, Hans1, Splinter, Erik1, Wijchers, Patrick J.1, Krijger, Peter H.L.1, de Laat, Wouter1 w.delaat@hubrecht.eu |
| المصدر: |
Molecular Cell. Nov2015, Vol. 60 Issue 4, p676-684. 9p. |
| مصطلحات موضوعية: |
*TRANSCRIPTIONAL repressor CTCF, *CELL polarity, *MOLECULAR structure of chromatin, *BINDING sites, *GENOME editing, *COHESINS |
| مستخلص: |
Summary CCCTC-binding factor (CTCF) is an architectural protein involved in the three-dimensional (3D) organization of chromatin. In this study, we assayed the 3D genomic contact profiles of a large number of CTCF binding sites with high-resolution 4C-seq. As recently reported, our data also suggest that chromatin loops preferentially form between CTCF binding sites oriented in a convergent manner. To directly test this, we used CRISPR/Cas9 genome editing to delete core CTCF binding sites in three loci, including the CTCF site in the Sox2 super-enhancer. In all instances, CTCF and cohesin recruitment were lost, and chromatin loops with distal, convergent CTCF sites were disrupted or destabilized. Re-insertion of oppositely oriented CTCF recognition sequences restored CTCF and cohesin recruitment, but did not re-establish chromatin loops. We conclude that CTCF binding polarity plays a functional role in the formation of higher-order chromatin structure. [ABSTRACT FROM AUTHOR] |
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