التفاصيل البيبلوغرافية
| العنوان: |
Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids. |
| المؤلفون: |
Kratochwill, Klaus1,2 klaus.kratochwill@meduniwien.ac.at, Bender, Thorsten O.1,3 tobender@web.de, Lichtenauer, Anton M.1,2 a.lichtenauer@zytoprotec.com, Herzog, Rebecca1,2 rebecca.herzog@meduniwien.ac.at, Tarantino, Silvia1 silvia.tarantino@meduniwien.ac.at, Bialas, Katarzyna2 k.bialas@zytoprotec.com, Jörres, Achim3 achim.joerres@charite.de, Aufricht, Christoph1 christoph.aufricht@meduniwien.ac.at |
| المصدر: |
BioMed Research International. 10/1/2015, Vol. 2015, p1-12. 12p. |
| مصطلحات موضوعية: |
*PERITONEAL dialysis, *CYTOPROTECTION, *HEAT shock proteins, *GEL electrophoresis, *GENE expression, *WESTERN immunoblotting, *CYTOTOXINS, *MESSENGER RNA, *BODY fluids, *ELECTROPHORESIS, *PSYCHOLOGICAL stress, *GENE expression profiling |
| مستخلص: |
Recent research suggests that cytoprotective responses, such as expression of heat-shock proteins, might be inadequately induced in mesothelial cells by heat-sterilized peritoneal dialysis (PD) fluids. This study compares transcriptome data and multiple protein expression profiles for providing new insight into regulatory mechanisms. Two-dimensional difference gel electrophoresis (2D-DIGE) based proteomics and topic defined gene expression microarray-based transcriptomics techniques were used to evaluate stress responses in human omental peritoneal mesothelial cells in response to heat- or filter-sterilized PD fluids. Data from selected heat-shock proteins were validated by 2D western-blot analysis. Comparison of proteomics and transcriptomics data discriminated differentially regulated protein abundance into groups depending on correlating or noncorrelating transcripts. Inadequate abundance of several heat-shock proteins following exposure to heat-sterilized PD fluids is not reflected on the mRNA level indicating interference beyond transcriptional regulation. For the first time, this study describes evidence for posttranscriptional inadequacy of heat-shock protein expression by heat-sterilized PD fluids as a novel cytotoxic property. Cross-omics technologies introduce a novel way of understanding PDF bioincompatibility and searching for new interventions to reestablish adequate cytoprotective responses. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder