التفاصيل البيبلوغرافية
| العنوان: |
MicroRNA Expression Profiling and DNA Methylation Signature for Deregulated MicroRNA in Cutaneous T-Cell Lymphoma. |
| المؤلفون: |
Sandoval, Juan1, Díaz-Lagares, Angel1, Salgado, Rocío2, Servitje, Octavio3, Climent, Fina3, Ortiz-Romero, Pablo L4, Pérez-Ferriols, Amparo5, Garcia-Muret, Maria P6, Estrach, Teresa7, Garcia, Mar2, Nonell, Lara8, Esteller, Manel9, Pujol, Ramon M10, Espinet, Blanca11, Gallardo, Fernando12 |
| المصدر: |
Journal of Investigative Dermatology. Apr2015, Vol. 135 Issue 4, p1128-1137. 10p. |
| مصطلحات موضوعية: |
*T-cell lymphoma, *MICRORNA, *GENETIC transcription, *DNA methylation, *GENETIC regulation, *MICROARRAY technology |
| مستخلص: |
MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients. [ABSTRACT FROM AUTHOR] |
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