دورية أكاديمية
Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
| العنوان: | Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua |
|---|---|
| المؤلفون: | Virginia Chu Cheung, Chian-Yu Peng, Mirna Marinić, Noboru J. Sakabe, Ivy Aneas, Vincent J. Lynch, Carole Ober, Marcelo A. Nobrega, John A. Kessler |
| المصدر: | Cell Reports, Vol 35, Iss 7, Pp 109138- (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | human pluripotent stem cells, endometrial stromal cells, decidua, epithelial-stromal signaling, cyclic hormone response, cell-cell signaling, Biology (General), QH301-705.5 |
| الوصف: | Summary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interactions between endometrial stromal and epithelial compartments necessary for decidualization and implantation. Here we report a protocol differentiating human pluripotent stem cells into endometrial stromal fibroblasts (PSC-ESFs) that are highly pure and able to decidualize. Coculture of PSC-ESFs with placenta-derived endometrial epithelial cells generated organoids used to examine stromal-epithelial interactions. Cocultures exhibited specific endometrial markers in the appropriate compartments, organization with cell polarity, and hormone responsiveness of both cell types. Furthermore, cocultures recapitulate a central feature of the human decidua by cyclically responding to hormone withdrawal followed by hormone retreatment. This advance enables mechanistic studies of the cyclic responses that characterize the human endometrium. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2211-1247 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2211124721004770Test; https://doaj.org/toc/2211-1247Test |
| DOI: | 10.1016/j.celrep.2021.109138 |
| الوصول الحر: | https://doaj.org/article/f12da9fe6d524cb6bd0633fc2545a92bTest |
| رقم الانضمام: | edsdoj.f12da9fe6d524cb6bd0633fc2545a92b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edsdoj Directory of Open Access Journals edsdoj.f12da9fe6d524cb6bd0633fc2545a92b 936 3 Academic Journal academicJournal 936.322387695313 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.f12da9fe6d524cb6bd0633fc2545a92b&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/f12da9fe6d524cb6bd0633fc2545a92b [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=22111247&ISBN=&volume=35&issue=7&date=20210501&spage=&pages=&title=Cell Reports&atitle=Pluripotent%20stem%20cell-derived%20endometrial%20stromal%20fibroblasts%20in%20a%20cyclic%2C%20hormone-responsive%2C%20coculture%20model%20of%20human%20decidua&id=DOI:10.1016/j.celrep.2021.109138 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Virginia+Chu+Cheung%22">Virginia Chu Cheung</searchLink><br /><searchLink fieldCode="AR" term="%22Chian-Yu+Peng%22">Chian-Yu Peng</searchLink><br /><searchLink fieldCode="AR" term="%22Mirna+Marinić%22">Mirna Marinić</searchLink><br /><searchLink fieldCode="AR" term="%22Noboru+J%2E+Sakabe%22">Noboru J. Sakabe</searchLink><br /><searchLink fieldCode="AR" term="%22Ivy+Aneas%22">Ivy Aneas</searchLink><br /><searchLink fieldCode="AR" term="%22Vincent+J%2E+Lynch%22">Vincent J. Lynch</searchLink><br /><searchLink fieldCode="AR" term="%22Carole+Ober%22">Carole Ober</searchLink><br /><searchLink fieldCode="AR" term="%22Marcelo+A%2E+Nobrega%22">Marcelo A. Nobrega</searchLink><br /><searchLink fieldCode="AR" term="%22John+A%2E+Kessler%22">John A. Kessler</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Cell Reports, Vol 35, Iss 7, Pp 109138- (2021) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Elsevier, 2021. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2021 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Biology (General) ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22human+pluripotent+stem+cells%22">human pluripotent stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22endometrial+stromal+cells%22">endometrial stromal cells</searchLink><br /><searchLink fieldCode="DE" term="%22decidua%22">decidua</searchLink><br /><searchLink fieldCode="DE" term="%22epithelial-stromal+signaling%22">epithelial-stromal signaling</searchLink><br /><searchLink fieldCode="DE" term="%22cyclic+hormone+response%22">cyclic hormone response</searchLink><br /><searchLink fieldCode="DE" term="%22cell-cell+signaling%22">cell-cell signaling</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Summary: Various human diseases and pregnancy-related disorders reflect endometrial dysfunction. However, rodent models do not share fundamental biological processes with the human endometrium, such as spontaneous decidualization, and no existing human cell cultures recapitulate the cyclic interactions between endometrial stromal and epithelial compartments necessary for decidualization and implantation. Here we report a protocol differentiating human pluripotent stem cells into endometrial stromal fibroblasts (PSC-ESFs) that are highly pure and able to decidualize. Coculture of PSC-ESFs with placenta-derived endometrial epithelial cells generated organoids used to examine stromal-epithelial interactions. Cocultures exhibited specific endometrial markers in the appropriate compartments, organization with cell polarity, and hormone responsiveness of both cell types. Furthermore, cocultures recapitulate a central feature of the human decidua by cyclically responding to hormone withdrawal followed by hormone retreatment. This advance enables mechanistic studies of the cyclic responses that characterize the human endometrium. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 2211-1247 ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => http://www.sciencedirect.com/science/article/pii/S2211124721004770; https://doaj.org/toc/2211-1247 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1016/j.celrep.2021.109138 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/f12da9fe6d524cb6bd0633fc2545a92b" linkWindow="_blank">https://doaj.org/article/f12da9fe6d524cb6bd0633fc2545a92b</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.f12da9fe6d524cb6bd0633fc2545a92b ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1016/j.celrep.2021.109138
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => human pluripotent stem cells
[Type] => general
)
[1] => Array
(
[SubjectFull] => endometrial stromal cells
[Type] => general
)
[2] => Array
(
[SubjectFull] => decidua
[Type] => general
)
[3] => Array
(
[SubjectFull] => epithelial-stromal signaling
[Type] => general
)
[4] => Array
(
[SubjectFull] => cyclic hormone response
[Type] => general
)
[5] => Array
(
[SubjectFull] => cell-cell signaling
[Type] => general
)
[6] => Array
(
[SubjectFull] => Biology (General)
[Type] => general
)
[7] => Array
(
[SubjectFull] => QH301-705.5
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Virginia Chu Cheung
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Chian-Yu Peng
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Mirna Marinić
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Noboru J. Sakabe
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ivy Aneas
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Vincent J. Lynch
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Carole Ober
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Marcelo A. Nobrega
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => John A. Kessler
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 05
[Type] => published
[Y] => 2021
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 22111247
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 35
)
[1] => Array
(
[Type] => issue
[Value] => 7
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Cell Reports
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |