دورية أكاديمية
Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway
| العنوان: | Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway |
|---|---|
| المؤلفون: | LIAO Min, LI Xuan, ZHANG Liangjun, SHI Pan, CHAI Jin |
| المصدر: | Di-san junyi daxue xuebao, Vol 43, Iss 12, Pp 1133-1139 (2021) |
| بيانات النشر: | Editorial Office of Journal of Third Military Medical University, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | artesunate, conjugated bile acids, cholestasis, primary mouse hepatocytes, il-6, Medicine (General), R5-920 |
| الوصف: | Objective To investigate the effect and underlying mechanism of artesunate (AS) on the expression of interleukin-6 (IL-6) in mouse primary hepatocytes induced by conjugate bile acids. Methods Mouse hepatocytes were isolated and primarily cultured, and then treated with 25 μmol/L conjugated bile acids. CCK-8 assay was used to determine the toxic effects of AS on the liver cells. The mRNA level of IL-6 in the cells was measured by RT-qPCR, and the protein levels of IL-6, p-65 and phosphorylated-p65 (p-p65) were determined by Western blotting. Results Conjugated bile acids of 25 μmol/L significantly promoted the expression of IL-6 at mRNA and protein levels in mouse primary hepatocytes, while, AS reduced the taurocholic acid (TCA)-induced phosphorylation of p65 and IL-6 level. Further studies revealed that AS suppressed the IL-6 expression through inhibiting p-p65 signaling pathway in mouse primary hepatocytes. Conclusion AS reduces IL-6 expression in mouse primary hepatocytes induced by conjugate bile acids via inhibiting the p-p65 signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Chinese |
| تدمد: | 1000-5404 |
| العلاقة: | http://aammt.tmmu.edu.cn/Upload/rhtml/202012225.htmTest; https://doaj.org/toc/1000-5404Test |
| DOI: | 10.16016/j.1000-5404.202012225 |
| الوصول الحر: | https://doaj.org/article/d9f920796abe41ba9ac946ac8890d736Test |
| رقم الانضمام: | edsdoj.9f920796abe41ba9ac946ac8890d736 |
| قاعدة البيانات: | Directory of Open Access Journals |
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