دورية أكاديمية
Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA
| العنوان: | Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA |
|---|---|
| المؤلفون: | Han Wang, Antonia Margarethe Stehr, Jeeshan Singh, Leticija Zlatar, Arndt Hartmann, Katja Evert, Elisabeth Naschberger, Saskia von Stillfried, Peter Boor, Luis E. Muñoz, Jasmin Knopf, Michael Stürzl, Martin Herrmann |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 4, p 4101 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | extracellular DNA, NETs, anti-DNA-IgM, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | During inflammatory responses, neutrophils enter the sites of attack where they execute various defense mechanisms. They (I) phagocytose microorganisms, (II) degranulate to release cytokines, (III) recruit various immune cells by cell-type specific chemokines, (IV) secrete anti-microbials including lactoferrin, lysozyme, defensins and reactive oxygen species, and (V) release DNA as neutrophil extracellular traps (NETs). The latter originates from mitochondria as well as from decondensed nuclei. This is easily detected in cultured cells by staining of DNA with specific dyes. However, in tissues sections the very high fluorescence signals emitted from the condensed nuclear DNA hamper the detection of the widespread, extranuclear DNA of the NETs. In contrast, when we employ anti-DNA-IgM antibodies, they are unable to penetrate deep into the tightly packed DNA of the nucleus, and we observe a robust signal for the extended DNA patches of the NETs. To validate anti-DNA-IgM, we additionally stained the sections for the NET-markers histone H2B, myeloperoxidase, citrullinated histone H3, and neutrophil elastase. Altogether, we have described a fast one-step procedure for the detection of NETs in tissue sections, which provides new perspectives to characterize neutrophil-associated immune reactions in disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/24/4/4101Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms24044101 |
| الوصول الحر: | https://doaj.org/article/8fba3635a58d40c6aaf459ee06b1d3aeTest |
| رقم الانضمام: | edsdoj.8fba3635a58d40c6aaf459ee06b1d3ae |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edsdoj Directory of Open Access Journals edsdoj.8fba3635a58d40c6aaf459ee06b1d3ae 958 3 Academic Journal academicJournal 958.036926269531 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.8fba3635a58d40c6aaf459ee06b1d3ae&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/8fba3635a58d40c6aaf459ee06b1d3ae [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=14220067&ISBN=&volume=24&issue=4&date=20230201&spage=4101&pages=4101-4101&title=International Journal of Molecular Sciences&atitle=Anti-DNA-IgM%20Favors%20the%20Detection%20of%20NET-Associated%20Extracellular%20DNA&id=DOI:10.3390/ijms24044101 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Han+Wang%22">Han Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Antonia+Margarethe+Stehr%22">Antonia Margarethe Stehr</searchLink><br /><searchLink fieldCode="AR" term="%22Jeeshan+Singh%22">Jeeshan Singh</searchLink><br /><searchLink fieldCode="AR" term="%22Leticija+Zlatar%22">Leticija Zlatar</searchLink><br /><searchLink fieldCode="AR" term="%22Arndt+Hartmann%22">Arndt Hartmann</searchLink><br /><searchLink fieldCode="AR" term="%22Katja+Evert%22">Katja Evert</searchLink><br /><searchLink fieldCode="AR" term="%22Elisabeth+Naschberger%22">Elisabeth Naschberger</searchLink><br /><searchLink fieldCode="AR" term="%22Saskia+von+Stillfried%22">Saskia von Stillfried</searchLink><br /><searchLink fieldCode="AR" term="%22Peter+Boor%22">Peter Boor</searchLink><br /><searchLink fieldCode="AR" term="%22Luis+E%2E+Muñoz%22">Luis E. Muñoz</searchLink><br /><searchLink fieldCode="AR" term="%22Jasmin+Knopf%22">Jasmin Knopf</searchLink><br /><searchLink fieldCode="AR" term="%22Michael+Stürzl%22">Michael Stürzl</searchLink><br /><searchLink fieldCode="AR" term="%22Martin+Herrmann%22">Martin Herrmann</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => International Journal of Molecular Sciences, Vol 24, Iss 4, p 4101 (2023) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => MDPI AG, 2023. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2023 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Biology (General)<br />LCC:Chemistry ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22extracellular+DNA%22">extracellular DNA</searchLink><br /><searchLink fieldCode="DE" term="%22NETs%22">NETs</searchLink><br /><searchLink fieldCode="DE" term="%22anti-DNA-IgM%22">anti-DNA-IgM</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22QD1-999%22">QD1-999</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => During inflammatory responses, neutrophils enter the sites of attack where they execute various defense mechanisms. They (I) phagocytose microorganisms, (II) degranulate to release cytokines, (III) recruit various immune cells by cell-type specific chemokines, (IV) secrete anti-microbials including lactoferrin, lysozyme, defensins and reactive oxygen species, and (V) release DNA as neutrophil extracellular traps (NETs). The latter originates from mitochondria as well as from decondensed nuclei. This is easily detected in cultured cells by staining of DNA with specific dyes. However, in tissues sections the very high fluorescence signals emitted from the condensed nuclear DNA hamper the detection of the widespread, extranuclear DNA of the NETs. In contrast, when we employ anti-DNA-IgM antibodies, they are unable to penetrate deep into the tightly packed DNA of the nucleus, and we observe a robust signal for the extended DNA patches of the NETs. To validate anti-DNA-IgM, we additionally stained the sections for the NET-markers histone H2B, myeloperoxidase, citrullinated histone H3, and neutrophil elastase. Altogether, we have described a fast one-step procedure for the detection of NETs in tissue sections, which provides new perspectives to characterize neutrophil-associated immune reactions in disease. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 1422-0067<br />1661-6596 ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://www.mdpi.com/1422-0067/24/4/4101; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.3390/ijms24044101 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/8fba3635a58d40c6aaf459ee06b1d3ae" linkWindow="_blank">https://doaj.org/article/8fba3635a58d40c6aaf459ee06b1d3ae</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.8fba3635a58d40c6aaf459ee06b1d3ae ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3390/ijms24044101
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 1
[StartPage] => 4101
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => extracellular DNA
[Type] => general
)
[1] => Array
(
[SubjectFull] => NETs
[Type] => general
)
[2] => Array
(
[SubjectFull] => anti-DNA-IgM
[Type] => general
)
[3] => Array
(
[SubjectFull] => Biology (General)
[Type] => general
)
[4] => Array
(
[SubjectFull] => QH301-705.5
[Type] => general
)
[5] => Array
(
[SubjectFull] => Chemistry
[Type] => general
)
[6] => Array
(
[SubjectFull] => QD1-999
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Han Wang
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Antonia Margarethe Stehr
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jeeshan Singh
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Leticija Zlatar
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Arndt Hartmann
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Katja Evert
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Elisabeth Naschberger
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Saskia von Stillfried
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Peter Boor
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Luis E. Muñoz
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jasmin Knopf
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Michael Stürzl
)
)
)
[12] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Martin Herrmann
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 02
[Type] => published
[Y] => 2023
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 14220067
)
[1] => Array
(
[Type] => issn-print
[Value] => 16616596
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 24
)
[1] => Array
(
[Type] => issue
[Value] => 4
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => International Journal of Molecular Sciences
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |