دورية أكاديمية
Cellular vimentin regulates the infectivity of Newcastle disease virus through targeting of the HN protein
| العنوان: | Cellular vimentin regulates the infectivity of Newcastle disease virus through targeting of the HN protein |
|---|---|
| المؤلفون: | Xiaolong Lu, Kaituo Liu, Yu Chen, Ruyi Gao, Zenglei Hu, Jiao Hu, Min Gu, Shunlin Hu, Chan Ding, Xinan Jiao, Xiaoquan Wang, Xiufan Liu, Xiaowen Liu |
| المصدر: | Veterinary Research, Vol 54, Iss 1, Pp 1-17 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | Newcastle disease virus, HN, vimentin, viral infection, macrophage, Veterinary medicine, SF600-1100 |
| الوصف: | Abstract The haemagglutinin-neuraminidase (HN) protein plays a crucial role in the infectivity and virulence of Newcastle disease virus (NDV). In a previous study, the mutant HN protein was identified as a crucial virulence factor for the velogenic variant NDV strain JS/7/05/Ch, which evolved from the prototypic vaccine strain Mukteswar. Furthermore, macrophages are the main susceptible target cells of NDV. However, the possible involvement of cellular molecules in viral infectivity remains unclear. Herein, we elucidate the crucial role of vimentin, an intermediate filament protein, in regulating NDV infectivity through targeting of the HN protein. Using LC‒MS/MS mass spectrometry and coimmunoprecipitation assays, we identified vimentin as a host protein that differentially interacted with prototypic and mutant HN proteins. Further analysis revealed that the variant NDV strain induced more significant rearrangement of vimentin fibres compared to the prototypic NDV strain and showed an interdependence between vimentin rearrangement and virus replication. Notably, these mutual influences were pronounced in HD11 chicken macrophages. Moreover, vimentin was required for multiple infection processes of the variant NDV strain in HD11 cells, including viral internalization, fusion, and release, while it was not necessary for those of the prototypic NDV strain. Collectively, these findings underscore the pivotal role of vimentin in NDV infection through targeting of the HN protein, providing novel targets for antiviral treatment strategies for NDV. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1297-9716 |
| العلاقة: | https://doaj.org/toc/1297-9716Test |
| DOI: | 10.1186/s13567-023-01230-5 |
| الوصول الحر: | https://doaj.org/article/87bd018d1cad4bb7a588b43e8cb2dd13Test |
| رقم الانضمام: | edsdoj.87bd018d1cad4bb7a588b43e8cb2dd13 |
| قاعدة البيانات: | Directory of Open Access Journals |
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