دورية أكاديمية
Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats
العنوان: | Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats |
---|---|
المؤلفون: | Quan-Bing Zhang, Lei Huo, Mian Li, Rui Zhang, Ting Zhou, Feng Wang, Yun Zhou |
المصدر: | European Journal of Medical Research, Vol 29, Iss 1, Pp 1-12 (2024) |
بيانات النشر: | BMC, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Medicine |
مصطلحات موضوعية: | Joint contracture, Joint capsule fibrosis, HIF-1α/NLRP3 signaling pathway, Pyroptosis, TGF-β1/Smad3 signaling pathway, Medicine |
الوصف: | Abstract Joint contracture is one of the common diseases clinically, and joint capsule fibrosis is considered to be one of the most important pathological changes of joint contracture. However, the underlying mechanism of joint capsule fibrosis is still controversial. The present study aims to establish an animal model of knee extending joint contracture in rats, and to investigate the role of hypoxia-mediated pyroptosis in the progression of joint contracture using this animal model. 36 male SD rats were selected, 6 of which were not immobilized and were used as control group, while 30 rats were divided into I-1 group (immobilized for 1 week following 7 weeks of free movement), I-2 group (immobilized for 2 weeks following 6 weeks of free movement), I-4 group (immobilized for 4 weeks following 4 weeks of free movement), I-6 group (immobilized for 6 weeks following 2 weeks of free movement) and I-8 group (immobilized for 8 weeks) according to different immobilizing time. The progression of joint contracture was assessed by the measurement of knee joint range of motion, collagen deposition in joint capsule was examined with Masson staining, protein expression levels of HIF-1α, NLRP3, Caspase-1, GSDMD-N, TGF-β1, α-SMA and p-Smad3 in joint capsule were assessed using western blotting, and the morphological changes of fibroblasts were observed by transmission electron microscopy. The degree of total and arthrogenic contracture progressed from the first week and lasted until the first eight weeks after immobilization. The degree of total and arthrogenic contracture progressed rapidly in the first four weeks after immobilization and then progressed slowly. Masson staining indicated that collagen deposition in joint capsule gradually increased in the first 8 weeks following immobilization. Western blotting analysis showed that the protein levels of HIF-1α continued to increase during the first 8 weeks of immobilization, and the protein levels of pyroptosis-related proteins NLRP3, Caspase-1, GSDMD-N continued to increase in the first 4 weeks after immobilization and then decreased. The protein levels of fibrosis-related proteins TGF-β1, p-Smad3 and α-SMA continued to increase in the first 8 weeks after immobilization. Transmission electron microscopy showed that 4 weeks of immobilization induced cell membrane rupture and cell contents overflow, which further indicated the activation of pyroptosis. Knee extending joint contracture animal model can be established by external immobilization orthosis in rats, and the activation of hypoxia-mediated pyroptosis may play a stimulating role in the process of joint capsule fibrosis and joint contracture. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2047-783X |
العلاقة: | https://doaj.org/toc/2047-783XTest |
DOI: | 10.1186/s40001-024-01890-9 |
الوصول الحر: | https://doaj.org/article/7e4cddea11d0411ca9f8663727ec06ebTest |
رقم الانضمام: | edsdoj.7e4cddea11d0411ca9f8663727ec06eb |
قاعدة البيانات: | Directory of Open Access Journals |
ResultId |
1 |
---|---|
Header |
edsdoj Directory of Open Access Journals edsdoj.7e4cddea11d0411ca9f8663727ec06eb 1080 3 Academic Journal academicJournal 1080.27294921875 |
PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.7e4cddea11d0411ca9f8663727ec06eb&custid=s6537998&authtype=sso |
FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/7e4cddea11d0411ca9f8663727ec06eb [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=2047783X&ISBN=&volume=29&issue=1&date=20240501&spage=1&pages=1-12&title=European Journal of Medical Research&atitle=Role%20of%20hypoxia-mediated%20pyroptosis%20in%20the%20development%20of%20extending%20knee%20joint%20contracture%20in%20rats&id=DOI:10.1186/s40001-024-01890-9 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Quan-Bing+Zhang%22">Quan-Bing Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Lei+Huo%22">Lei Huo</searchLink><br /><searchLink fieldCode="AR" term="%22Mian+Li%22">Mian Li</searchLink><br /><searchLink fieldCode="AR" term="%22Rui+Zhang%22">Rui Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Ting+Zhou%22">Ting Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Feng+Wang%22">Feng Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Yun+Zhou%22">Yun Zhou</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => European Journal of Medical Research, Vol 29, Iss 1, Pp 1-12 (2024) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => BMC, 2024. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2024 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Medicine ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Joint+contracture%22">Joint contracture</searchLink><br /><searchLink fieldCode="DE" term="%22Joint+capsule+fibrosis%22">Joint capsule fibrosis</searchLink><br /><searchLink fieldCode="DE" term="%22HIF-1α%2FNLRP3+signaling+pathway%22">HIF-1α/NLRP3 signaling pathway</searchLink><br /><searchLink fieldCode="DE" term="%22Pyroptosis%22">Pyroptosis</searchLink><br /><searchLink fieldCode="DE" term="%22TGF-β1%2FSmad3+signaling+pathway%22">TGF-β1/Smad3 signaling pathway</searchLink><br /><searchLink fieldCode="DE" term="%22Medicine%22">Medicine</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Abstract Joint contracture is one of the common diseases clinically, and joint capsule fibrosis is considered to be one of the most important pathological changes of joint contracture. However, the underlying mechanism of joint capsule fibrosis is still controversial. The present study aims to establish an animal model of knee extending joint contracture in rats, and to investigate the role of hypoxia-mediated pyroptosis in the progression of joint contracture using this animal model. 36 male SD rats were selected, 6 of which were not immobilized and were used as control group, while 30 rats were divided into I-1 group (immobilized for 1 week following 7 weeks of free movement), I-2 group (immobilized for 2 weeks following 6 weeks of free movement), I-4 group (immobilized for 4 weeks following 4 weeks of free movement), I-6 group (immobilized for 6 weeks following 2 weeks of free movement) and I-8 group (immobilized for 8 weeks) according to different immobilizing time. The progression of joint contracture was assessed by the measurement of knee joint range of motion, collagen deposition in joint capsule was examined with Masson staining, protein expression levels of HIF-1α, NLRP3, Caspase-1, GSDMD-N, TGF-β1, α-SMA and p-Smad3 in joint capsule were assessed using western blotting, and the morphological changes of fibroblasts were observed by transmission electron microscopy. The degree of total and arthrogenic contracture progressed from the first week and lasted until the first eight weeks after immobilization. The degree of total and arthrogenic contracture progressed rapidly in the first four weeks after immobilization and then progressed slowly. Masson staining indicated that collagen deposition in joint capsule gradually increased in the first 8 weeks following immobilization. Western blotting analysis showed that the protein levels of HIF-1α continued to increase during the first 8 weeks of immobilization, and the protein levels of pyroptosis-related proteins NLRP3, Caspase-1, GSDMD-N continued to increase in the first 4 weeks after immobilization and then decreased. The protein levels of fibrosis-related proteins TGF-β1, p-Smad3 and α-SMA continued to increase in the first 8 weeks after immobilization. Transmission electron microscopy showed that 4 weeks of immobilization induced cell membrane rupture and cell contents overflow, which further indicated the activation of pyroptosis. Knee extending joint contracture animal model can be established by external immobilization orthosis in rats, and the activation of hypoxia-mediated pyroptosis may play a stimulating role in the process of joint capsule fibrosis and joint contracture. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 2047-783X ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://doaj.org/toc/2047-783X ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1186/s40001-024-01890-9 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/7e4cddea11d0411ca9f8663727ec06eb" linkWindow="_blank">https://doaj.org/article/7e4cddea11d0411ca9f8663727ec06eb</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.7e4cddea11d0411ca9f8663727ec06eb ) |
RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1186/s40001-024-01890-9
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[PhysicalDescription] => Array
(
[Pagination] => Array
(
[PageCount] => 12
[StartPage] => 1
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Joint contracture
[Type] => general
)
[1] => Array
(
[SubjectFull] => Joint capsule fibrosis
[Type] => general
)
[2] => Array
(
[SubjectFull] => HIF-1α/NLRP3 signaling pathway
[Type] => general
)
[3] => Array
(
[SubjectFull] => Pyroptosis
[Type] => general
)
[4] => Array
(
[SubjectFull] => TGF-β1/Smad3 signaling pathway
[Type] => general
)
[5] => Array
(
[SubjectFull] => Medicine
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Quan-Bing Zhang
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lei Huo
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Mian Li
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rui Zhang
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ting Zhou
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Feng Wang
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yun Zhou
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 05
[Type] => published
[Y] => 2024
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 2047783X
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 29
)
[1] => Array
(
[Type] => issue
[Value] => 1
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => European Journal of Medical Research
[Type] => main
)
)
)
)
)
)
)
|
IllustrationInfo |