دورية أكاديمية
T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B
| العنوان: | T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B |
|---|---|
| المؤلفون: | Agustin Garcia-Caballero, Fang-Xiong Zhang, Victoria Hodgkinson, Junting Huang, Lina Chen, Ivana A. Souza, Stuart Cain, Jennifer Kass, Sascha Alles, Terrance P. Snutch, Gerald W. Zamponi |
| المصدر: | Molecular Brain, Vol 11, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: | T-type channels, Spectrin (α/β), Ankyrin B, Trafficking, Cav3.1, Cav3.2, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | Abstract This study describes the functional interaction between the Cav3.1 and Cav3.2 T-type calcium channels and cytoskeletal spectrin (α/β) and ankyrin B proteins. The interactions were identified utilizing a proteomic approach to identify proteins that interact with a conserved negatively charged cytosolic region present in the carboxy-terminus of T-type calcium channels. Deletion of this stretch of amino acids decreased binding of Cav3.1 and Cav3.2 calcium channels to spectrin (α/β) and ankyrin B and notably also reduced T-type whole cell current densities in expression systems. Furthermore, fluorescence recovery after photobleaching analysis of mutant channels lacking the proximal C-terminus region revealed reduced recovery of both Cav3.1 and Cav3.2 mutant channels in hippocampal neurons. Knockdown of spectrin α and ankyrin B decreased the density of endogenous Cav3.2 in hippocampal neurons. These findings reveal spectrin (α/β) / ankyrin B cytoskeletal and signaling proteins as key regulators of T-type calcium channels expressed in the nervous system. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-6606 |
| العلاقة: | http://link.springer.com/article/10.1186/s13041-018-0368-5Test; https://doaj.org/toc/1756-6606Test |
| DOI: | 10.1186/s13041-018-0368-5 |
| الوصول الحر: | https://doaj.org/article/ac77eff5edbc4bdca51628849a3670acTest |
| رقم الانضمام: | edsdoj.77eff5edbc4bdca51628849a3670ac |
| قاعدة البيانات: | Directory of Open Access Journals |
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