دورية أكاديمية
Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
| العنوان: | Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B |
|---|---|
| المؤلفون: | Pieter A Roelofs, Chai Yeen Goh, Boon Haow Chua, Matthew C Jarvis, Teneale A Stewart, Jennifer L McCann, Rebecca M McDougle, Michael A Carpenter, John WM Martens, Paul N Span, Dennis Kappei, Reuben S Harris |
| المصدر: | eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | APOBEC3B, cancer mutagenesis, DREAM complex, PRC1.6 complex, RB/E2F pathway, transcriptional regulation, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | APOBEC3B (A3B)-catalyzed DNA cytosine deamination contributes to the overall mutational landscape in breast cancer. Molecular mechanisms responsible for A3B upregulation in cancer are poorly understood. Here we show that a single E2F cis-element mediates repression in normal cells and that expression is activated by its mutational disruption in a reporter construct or the endogenous A3B gene. The same E2F site is required for A3B induction by polyomavirus T antigen indicating a shared molecular mechanism. Proteomic and biochemical experiments demonstrate the binding of wildtype but not mutant E2F promoters by repressive PRC1.6/E2F6 and DREAM/E2F4 complexes. Knockdown and overexpression studies confirm the involvement of these repressive complexes in regulating A3B expression. Altogether, these studies demonstrate that A3B expression is suppressed in normal cells by repressive E2F complexes and that viral or mutational disruption of this regulatory network triggers overexpression in breast cancer and provides fuel for tumor evolution. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/61287Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.61287 |
| الوصول الحر: | https://doaj.org/article/562661be3bbb4115a4d955575847fc12Test |
| رقم الانضمام: | edsdoj.562661be3bbb4115a4d955575847fc12 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | ResultId |
1 |
|---|---|
| Header |
edsdoj Directory of Open Access Journals edsdoj.562661be3bbb4115a4d955575847fc12 922 3 Academic Journal academicJournal 922.447143554688 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.562661be3bbb4115a4d955575847fc12&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doaj.org/article/562661be3bbb4115a4d955575847fc12 [Name] => EDS - DOAJ [Category] => fullText [Text] => View record in DOAJ [MouseOverText] => View record in DOAJ ) [1] => Array ( [Url] => https://resolver.ebscohost.com/openurl?custid=s6537998&groupid=main&authtype=ip,guest&sid=EBSCO:edsdoj&genre=article&issn=2050084X&ISBN=&volume=9&issue=&date=20200901&spage=&pages=&title=eLife&atitle=Characterization%20of%20the%20mechanism%20by%20which%20the%20RB%2FE2F%20pathway%20controls%20expression%20of%20the%20cancer%20genomic%20DNA%20deaminase%20APOBEC3B&id=DOI:10.7554/eLife.61287 [Name] => Full Text Finder (s6537998api) [Category] => fullText [Text] => Full Text Finder [Icon] => https://imageserver.ebscohost.com/branding/images/FTF.gif [MouseOverText] => Full Text Finder ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Pieter+A+Roelofs%22">Pieter A Roelofs</searchLink><br /><searchLink fieldCode="AR" term="%22Chai+Yeen+Goh%22">Chai Yeen Goh</searchLink><br /><searchLink fieldCode="AR" term="%22Boon+Haow+Chua%22">Boon Haow Chua</searchLink><br /><searchLink fieldCode="AR" term="%22Matthew+C+Jarvis%22">Matthew C Jarvis</searchLink><br /><searchLink fieldCode="AR" term="%22Teneale+A+Stewart%22">Teneale A Stewart</searchLink><br /><searchLink fieldCode="AR" term="%22Jennifer+L+McCann%22">Jennifer L McCann</searchLink><br /><searchLink fieldCode="AR" term="%22Rebecca+M+McDougle%22">Rebecca M McDougle</searchLink><br /><searchLink fieldCode="AR" term="%22Michael+A+Carpenter%22">Michael A Carpenter</searchLink><br /><searchLink fieldCode="AR" term="%22John+WM+Martens%22">John WM Martens</searchLink><br /><searchLink fieldCode="AR" term="%22Paul+N+Span%22">Paul N Span</searchLink><br /><searchLink fieldCode="AR" term="%22Dennis+Kappei%22">Dennis Kappei</searchLink><br /><searchLink fieldCode="AR" term="%22Reuben+S+Harris%22">Reuben S Harris</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => eLife, Vol 9 (2020) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => eLife Sciences Publications Ltd, 2020. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2020 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => LCC:Medicine<br />LCC:Science<br />LCC:Biology (General) ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22APOBEC3B%22">APOBEC3B</searchLink><br /><searchLink fieldCode="DE" term="%22cancer+mutagenesis%22">cancer mutagenesis</searchLink><br /><searchLink fieldCode="DE" term="%22DREAM+complex%22">DREAM complex</searchLink><br /><searchLink fieldCode="DE" term="%22PRC1%2E6+complex%22">PRC1.6 complex</searchLink><br /><searchLink fieldCode="DE" term="%22RB%2FE2F+pathway%22">RB/E2F pathway</searchLink><br /><searchLink fieldCode="DE" term="%22transcriptional+regulation%22">transcriptional regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Medicine%22">Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Science%22">Science</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+%28General%29%22">Biology (General)</searchLink><br /><searchLink fieldCode="DE" term="%22QH301-705%2E5%22">QH301-705.5</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => APOBEC3B (A3B)-catalyzed DNA cytosine deamination contributes to the overall mutational landscape in breast cancer. Molecular mechanisms responsible for A3B upregulation in cancer are poorly understood. Here we show that a single E2F cis-element mediates repression in normal cells and that expression is activated by its mutational disruption in a reporter construct or the endogenous A3B gene. The same E2F site is required for A3B induction by polyomavirus T antigen indicating a shared molecular mechanism. Proteomic and biochemical experiments demonstrate the binding of wildtype but not mutant E2F promoters by repressive PRC1.6/E2F6 and DREAM/E2F4 complexes. Knockdown and overexpression studies confirm the involvement of these repressive complexes in regulating A3B expression. Altogether, these studies demonstrate that A3B expression is suppressed in normal cells by repressive E2F complexes and that viral or mutational disruption of this regulatory network triggers overexpression in breast cancer and provides fuel for tumor evolution. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => electronic resource ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 2050-084X ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://elifesciences.org/articles/61287; https://doaj.org/toc/2050-084X ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.7554/eLife.61287 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://doaj.org/article/562661be3bbb4115a4d955575847fc12" linkWindow="_blank">https://doaj.org/article/562661be3bbb4115a4d955575847fc12</link> ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsdoj.562661be3bbb4115a4d955575847fc12 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.7554/eLife.61287
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => APOBEC3B
[Type] => general
)
[1] => Array
(
[SubjectFull] => cancer mutagenesis
[Type] => general
)
[2] => Array
(
[SubjectFull] => DREAM complex
[Type] => general
)
[3] => Array
(
[SubjectFull] => PRC1.6 complex
[Type] => general
)
[4] => Array
(
[SubjectFull] => RB/E2F pathway
[Type] => general
)
[5] => Array
(
[SubjectFull] => transcriptional regulation
[Type] => general
)
[6] => Array
(
[SubjectFull] => Medicine
[Type] => general
)
[7] => Array
(
[SubjectFull] => Science
[Type] => general
)
[8] => Array
(
[SubjectFull] => Biology (General)
[Type] => general
)
[9] => Array
(
[SubjectFull] => QH301-705.5
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Pieter A Roelofs
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Chai Yeen Goh
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Boon Haow Chua
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Matthew C Jarvis
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Teneale A Stewart
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jennifer L McCann
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rebecca M McDougle
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Michael A Carpenter
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => John WM Martens
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Paul N Span
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Dennis Kappei
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Reuben S Harris
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 09
[Type] => published
[Y] => 2020
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-print
[Value] => 2050084X
)
)
[Numbering] => Array
(
[0] => Array
(
[Type] => volume
[Value] => 9
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => eLife
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |