دورية أكاديمية
Age-Dependent Changes in Calcium Regulation after Myocardial Ischemia–Reperfusion Injury
العنوان: | Age-Dependent Changes in Calcium Regulation after Myocardial Ischemia–Reperfusion Injury |
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المؤلفون: | Maria Bencurova, Terezia Lysikova, Katarina Leskova Majdova, Peter Kaplan, Peter Racay, Jan Lehotsky, Zuzana Tatarkova |
المصدر: | Biomedicines, Vol 11, Iss 4, p 1193 (2023) |
بيانات النشر: | MDPI AG, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | aging, heart, ischemia, reperfusion, Ca2+-ATPase, calcium handling, Biology (General), QH301-705.5 |
الوصف: | During aging, heart structure and function gradually deteriorate, which subsequently increases susceptibility to ischemia–reperfusion (IR). Maintenance of Ca2+ homeostasis is critical for cardiac contractility. We used Langendorff’s model to monitor the susceptibility of aging (6-, 15-, and 24-month-old) hearts to IR, with a specific focus on Ca2+-handling proteins. IR, but not aging itself, triggered left ventricular changes when the maximum rate of pressure development decreased in 24-month-olds, and the maximum rate of relaxation was most affected in 6-month-old hearts. Aging caused a deprivation of Ca2+-ATPase (SERCA2a), Na+/Ca2+ exchanger, mitochondrial Ca2+ uniporter, and ryanodine receptor contents. IR-induced damage to ryanodine receptor stimulates Ca2+ leakage in 6-month-old hearts and elevated phospholamban (PLN)-to-SERCA2a ratio can slow down Ca2+ reuptake seen at 2–5 μM Ca2+. Total and monomeric PLN mirrored the response of overexpressed SERCA2a after IR in 24-month-old hearts, resulting in stable Ca2+-ATPase activity. Upregulated PLN accelerated inhibition of Ca2+-ATPase activity at low free Ca2+ in 15-month-old after IR, and reduced SERCA2a content subsequently impairs the Ca2+-sequestering capacity. In conclusion, our study suggests that aging is associated with a significant decrease in the abundance and function of Ca2+-handling proteins. However, the IR-induced damage was not increased during aging. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2227-9059 |
العلاقة: | https://www.mdpi.com/2227-9059/11/4/1193Test; https://doaj.org/toc/2227-9059Test |
DOI: | 10.3390/biomedicines11041193 |
الوصول الحر: | https://doaj.org/article/4b248193d46b48d6b16fbf60d3a42cf0Test |
رقم الانضمام: | edsdoj.4b248193d46b48d6b16fbf60d3a42cf0 |
قاعدة البيانات: | Directory of Open Access Journals |
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