دورية أكاديمية
Leukocyte- and endothelial-derived microparticles: a circulating source for fibrinolysis
| العنوان: | Leukocyte- and endothelial-derived microparticles: a circulating source for fibrinolysis |
|---|---|
| المؤلفون: | Romaric Lacroix, Laurent Plawinski, Stéphane Robert, Loïc Doeuvre, Florence Sabatier, Sara Martinez de Lizarrondo, Anna Mezzapesa, Francine Anfosso, Aurelie S. Leroyer, Pascale Poullin, Noémie Jourde, Makon-Sébastien Njock, Chantal M. Boulanger, Eduardo Anglés-Cano, Françoise Dignat-George |
| المصدر: | Haematologica, Vol 97, Iss 12 (2012) |
| بيانات النشر: | Ferrata Storti Foundation, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: | Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: | Background We recently assigned a new fibrinolytic function to cell-derived microparticles in vitro. In this study we explored the relevance of this novel property of microparticles to the in vivo situation.Design and Methods Circulating microparticles were isolated from the plasma of patients with thrombotic thrombocytopenic purpura or cardiovascular disease and from healthy subjects. Microparticles were also obtained from purified human blood cell subpopulations. The plasminogen activators on microparticles were identified by flow cytometry and enzyme-linked immunosorbent assays; their capacity to generate plasmin was quantified with a chromogenic assay and their fibrinolytic activity was determined by zymography.Results Circulating microparticles isolated from patients generate a range of plasmin activity at their surface. This property was related to a variable content of urokinase-type plasminogen activator and/or tissue plasminogen activator. Using distinct microparticle subpopulations, we demonstrated that plasmin is generated on endothelial and leukocyte microparticles, but not on microparticles of platelet or erythrocyte origin. Leukocyte-derived microparticles bear urokinase-type plasminogen activator and its receptor whereas endothelial microparticles carry tissue plasminogen activator and tissue plasminogen activator/inhibitor complexes.Conclusions Endothelial and leukocyte microparticles, bearing respectively tissue plasminogen activator or urokinase-type plasminogen activator, support a part of the fibrinolytic activity in the circulation which is modulated in pathological settings. Awareness of this blood-borne fibrinolytic activity conveyed by microparticles provides a more comprehensive view of the role of microparticles in the hemostatic equilibrium. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0390-6078 1592-8721 |
| العلاقة: | https://haematologica.org/article/view/6502Test; https://doaj.org/toc/0390-6078Test; https://doaj.org/toc/1592-8721Test |
| DOI: | 10.3324/haematol.2012.066167 |
| الوصول الحر: | https://doaj.org/article/1b26f8a78f0e49bba17b73a3d7ae14c9Test |
| رقم الانضمام: | edsdoj.1b26f8a78f0e49bba17b73a3d7ae14c9 |
| قاعدة البيانات: | Directory of Open Access Journals |
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