دورية
Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
العنوان: | Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss) |
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المؤلفون: | Brzuzan, P., Woźny, M., Łuczyński, M. K., Góra, M., Ciesielski, S., Kuźmiński, H. |
المصدر: | Environmental Biotechnology. |
مصطلحات موضوعية: | benzo(a)piren, mózg, CYP1A, cyklopenta[c]fenantren, absolutna kwantyfikacja mRNA, pstrąg tęczowy, benzo(a)pyrene, brain, cyclopenta[c]phenanthrene, mRNA absolute quantification, rainbow trout |
الوصف: | We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure. |
نوع الوثيقة: | Article |
اللغة: | English |
الوصول الحر: | http://yadda.icm.edu.pl/baztech/element/bwmeta1.element.baztech-article-BAR0-0062-0095Test |
رقم الانضمام: | edsbzt.bwmeta1.element.baztech.article.BAR0.0062.0095 |
قاعدة البيانات: | BazTech |