دورية أكاديمية
Specific cleavage sites of Nef proteins from human immunodeficiency virus types 1 and 2 for the viral proteases
العنوان: | Specific cleavage sites of Nef proteins from human immunodeficiency virus types 1 and 2 for the viral proteases |
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المؤلفون: | Schorr, J, Kellner, R, Fackler, O, Freund, J, Konvalinka, J, Kienzle, N, Kräusslich, H G, Mueller-Lantzsch, N, Kalbitzer, H R |
المصدر: | Journal of Virology ; volume 70, issue 12, page 9051-9054 ; ISSN 0022-538X 1098-5514 |
بيانات النشر: | American Society for Microbiology |
سنة النشر: | 1996 |
الوصف: | Human immunodeficiency virus type 2 (HIV-2) Nef is proteolytically cleaved by the HIV-2-encoded protease. The proteolysis is not influenced by the absence or presence of the N-terminal myristoylation. The main cleavage site is located between residues 39 and 40, suggesting a protease recognition sequence, GGEY-SQFQ. As observed previously for Nef protein from HIV-1, a large, stable core domain with an apparent molecular mass of 30 kDa is produced by the proteolytic activity. Cleavage of Nef from HIV-1 in two domains by its own protease or the protease from HIV-2 is also independent of Nef myristoylation. However, processing of HIV-1 Nef by the HIV-2 protease is less selective than that by the HIV-1 protease: the obtained core fragment is heterogeneous at its N terminus and has an additional cleavage site between amino acids 99 and 100. Preliminary experiments suggest that the full-length Nef of HIV-2 and the core domain are part of the HIV-2 particles, analogous to the situation reported recently for HIV-1. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
DOI: | 10.1128/jvi.70.12.9051-9054.1996 |
الإتاحة: | https://doi.org/10.1128/jvi.70.12.9051-9054.1996Test |
حقوق: | https://journals.asm.org/non-commercial-tdm-licenseTest |
رقم الانضمام: | edsbas.F84BADB3 |
قاعدة البيانات: | BASE |