دورية أكاديمية
Cytosolic Ca2+ regulates the energization of isolated brain mitochondria by formation of pyruvate through the malate–aspartate shuttle
| العنوان: | Cytosolic Ca2+ regulates the energization of isolated brain mitochondria by formation of pyruvate through the malate–aspartate shuttle |
|---|---|
| المؤلفون: | Gellerich, Frank Norbert, Gizatullina, Zemfira, Trumbeckaitė, Sonata, Korzeniewski, Bernard, Gaynutdinov, Timur, Seppet, Enn K, Vielhaber, Stefan, Heinze, Hans-Jochen, Striggow, Frank |
| المصدر: | Biochemical journal, London, UK : Portland Press, 2012, vol. 443, no. 3, p. 747-755 ; ISSN 0264-6021 |
| سنة النشر: | 2012 |
| المجموعة: | LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) / LAEI VB (Lietuvos agrarinės ekonomikos institutasvirtualią biblioteką) |
| مصطلحات موضوعية: | Brain, Metabolism, Mitochondria, L-lactate dehydrogenase, Malates, Pyruvates, info:eu-repo/classification/udc/576.311.347 |
| الوصف: | The glutamate-dependent respiration of isolated BM (brain mitochondria) is regulated by Ca2+cyt (cytosolic Ca2+) (S0.5=225±22 nM) through its effects on aralar. We now also demonstrate that the α-glycerophosphate-dependent respiration is controlled by Ca2+cyt (S0.5=60±10 nM). At higher Ca2+cyt (>600 nM), BM accumulate Ca2+ which enhances the rate of intramitochondrial dehydrogenases. The Ca2+-induced increments of state 3 respiration decrease with substrate in the order glutamate>α-oxoglutarate>isocitrate>α-glycerophosphate>pyruvate. Whereas the oxidation of pyruvate is only slightly influenced by Ca2+cyt, we show that the formation of pyruvate is tightly controlled by Ca2+cyt. Through its common substrate couple NADH/NAD+, the formation of pyruvate by LDH (lactate dehydrogenase) is linked to the MAS (malate–aspartate shuttle) with aralar as a central component. A rise in Ca2+cyt in a reconstituted system consisting of BM, cytosolic enzymes of MAS and LDH causes an up to 5-fold enhancement of OXPHOS (oxidative phosphorylation) rates that is due to an increased substrate supply, acting in a manner similar to a ‘gas pedal’. In contrast, Ca2+mit (intramitochondrial Ca2+) regulates the oxidation rates of substrates which are present within the mitochondrial matrix. We postulate that Ca2+cyt is a key factor in adjusting the mitochondrial energization to the requirements of intact neurons. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://lsmu.lvb.lt/LSMU:ELABAPDB6186331&prefLang=en_USTest |
| الإتاحة: | https://doi.org/10.1042/BJ20110765Test http://lsmu.lvb.lt/LSMU:ELABAPDB6186331&prefLang=en_USTest |
| رقم الانضمام: | edsbas.D7BB1FEC |
| قاعدة البيانات: | BASE |
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