دورية أكاديمية
Achieving Ultrasensitive Chromogenic Probes for Rapid, Direct Detection of Carbapenemase-Producing Bacteria in Sputum
| العنوان: | Achieving Ultrasensitive Chromogenic Probes for Rapid, Direct Detection of Carbapenemase-Producing Bacteria in Sputum |
|---|---|
| المؤلفون: | Wenshuai Li (3430436), Jingjing Wang (140791), Chen Li (54018), Zhiyou Zong (7336904), Jinzhong Zhao (2175712), Hongmei Gao (240295), Dingbin Liu (1320243) |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Medicine, Microbiology, Genetics, Cancer, Infectious Diseases, Space Science, Biological Sciences not elsewhere classified, portable paper chip, effective infection management, complex molecular design, choosing strong electron, benzene derivative bearing, unexpectedly high sensitivity, clinical sputum samples, rapid cpb detection, clinical sensitivity, clinical samples, − n, withdrawing substituents, tunable substituent, sputum carbapenemase, sepsis patients, result time, readily performed, rapid point, quickly diagnosed, producing bacteria, modularly designed, lung infections, limited settings |
| الوصف: | Carbapenemase-producing bacteria (CPB) stand as the most dangerous “superbugs” in the clinic. Rapid point-of-care (POC) detection of CPB in clinical samples is key to timely and effective infection management. We herein report the first ultrasensitive chromogenic probe that allows direct POC detection of CPB in clinical sputum samples at a sample-to-result time of less than 15 min. This chromogenic probe is modularly designed by conjugating the carbapenem core with a benzene derivative bearing an electronegativity-tunable substituent. Unexpectedly high sensitivity was achieved simply by choosing strong electron-withdrawing substituents, such as −N + (CH 3 ) 3 , without resorting to complex molecular design. Through integrating the probes with a portable paper chip, 24 out of 80 clinical sputum samples from sepsis patients with lung infections were quickly diagnosed as CPB-positive, exhibiting 100% clinical sensitivity and specificity. This low-cost paper chip assay can be readily performed on-site, breaking through the dilemma of rapid CPB detection, especially in resource-limited settings. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Achieving_Ultrasensitive_Chromogenic_Probes_for_Rapid_Direct_Detection_of_Carbapenemase-Producing_Bacteria_in_Sputum/21782645Test |
| DOI: | 10.1021/jacsau.2c00607.s001 |
| الإتاحة: | https://doi.org/10.1021/jacsau.2c00607.s001Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.9084D77A |
| قاعدة البيانات: | BASE |
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