دورية أكاديمية
Natural Association of Lysozyme and Casein Micelles in Human Milk
| العنوان: | Natural Association of Lysozyme and Casein Micelles in Human Milk |
|---|---|
| المؤلفون: | Mathias Jaeser (12029944), Ulrike Moeckel (12029947), Kati Weigel (12029950), Thomas Henle (745885) |
| سنة النشر: | 2022 |
| المجموعة: | Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: | Biochemistry, Medicine, Microbiology, Cell Biology, Pharmacology, Immunology, Developmental Biology, Plant Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, performance liquid chromatography, flight mass spectrometry, 10 volunteering mothers, whey proteins, tof ), results indicate, natural association, micrococcus lysodeikticus, micellar structure, lytic activity, integral component, free protein, following ultracentifugation, enzymatic activity, electrospray ionization, cell walls, casein micelles, antibacterial activity, 96 μg |
| الوصف: | Using reversed phase high-performance liquid chromatography with ultraviolet (UV) detection and electrospray ionization (ESI)-quadrupole time-of-flight mass spectrometry (RP-HPLC-UV-ESI-Q-TOF), the lysozyme content in the milk of 10 volunteering mothers was quantified, ranging from 29 to 96 μg/mL. Following ultracentifugation, it was found that the lysozyme in human milk, unlike other whey proteins, is mainly bound to casein micelles (ca. 75%). The enzymatic activity of human lysozyme, measured as lytic activity against cell walls of Micrococcus lysodeikticus, was similar for the micelle-bound and free protein, indicating that the micellar structure should not affect the antibacterial activity of lysozyme. The results indicate that lysozyme is an integral component of casein micelles in human milk. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Natural_Association_of_Lysozyme_and_Casein_Micelles_in_Human_Milk/19104808Test |
| DOI: | 10.1021/acs.jafc.1c07192.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jafc.1c07192.s001Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.7D7E5AED |
| قاعدة البيانات: | BASE |
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