دورية أكاديمية
Unilateral immobilization: a simple model of limb atrophy in mice
| العنوان: | Unilateral immobilization: a simple model of limb atrophy in mice |
|---|---|
| المؤلفون: | Luca Madaro, Piera Smeriglio, Mario Molinaro, Marina Bouché |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.bio.unipd.it/~bam/PDF/18-5/Madaro.pdfTest. |
| المجموعة: | CiteSeerX |
| مصطلحات موضوعية: | Key Words, Muscle atrophy, muscle disuse, unilateral immobilization, MuRF1, atrogin-1 |
| الوصف: | We describe a new, simple immobilization protocol designed to induce muscle atrophy in lower hindlimb muscles in mice. Hindlimbs of C57BL6 mice were unilaterally immobilized for 7 days. Morphological analysis revealed that immobilization resulted in a significant reduction in muscle fiber size in both the extensor digitorum longus (EDL) and tibialis anterior (TA). By contrast, no significant changes in muscle fiber type content, as measured by NADH-TR staining, were detected. At the molecular level, expression of the muscle-specific ubiquitin ligases MAFbx/Atrogin-1 and MuRF1 genes was induced following immobilization in all the muscles examined. The characteristics of this model lend themselves to muscle atrophy research; its availability in mice may be instrumental for future studies on therapeutic interventions of muscle atrophy using transgenic and mutant mice strains. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.484.8265Test; http://www.bio.unipd.it/~bam/PDF/18-5/Madaro.pdfTest |
| الإتاحة: | http://www.bio.unipd.it/~bam/PDF/18-5/Madaro.pdfTest |
| حقوق: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.637045E5 |
| قاعدة البيانات: | BASE |
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