دورية أكاديمية
Investigation of baker's yeast Saccharomyces cerevisiae- and mediator-based carbon paste electrodes as amperometric biosensors for lactic acid
| العنوان: | Investigation of baker's yeast Saccharomyces cerevisiae- and mediator-based carbon paste electrodes as amperometric biosensors for lactic acid |
|---|---|
| المؤلفون: | Garjonytė, Rasa, Malinauskas, Albertas |
| المصدر: | Sensors and actuators. B, Chemical, 2003, Vol.96, Iss.3, p. 509-515 ; ISSN 0925-4005 |
| سنة النشر: | 2003 |
| المجموعة: | LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) / LAEI VB (Lietuvos agrarinės ekonomikos institutasvirtualią biblioteką) |
| مصطلحات موضوعية: | Lactate-dehydrogenase, Sensor, Oxidase, Enzyme, Immobilization, Bioelectrode, Oxidation, Sucrose, Films |
| الوصف: | Carbon paste electrodes modified with baker's yeast (a source of cytochrome b(2)) and various mediators (Nile blue, methylene blue, toluidine blue, Meldola blue, p-hydroquinone and Prussian Blue) were tested for the sensitivity to lactic acid. Electrochemical activity of mediators increased when baker's yeast was added to the paste. Only Meldola blue and Prussian Blue-modified electrodes responded to lactic acid. The operating potentials were +0.1 and +0.3 V (versus saturated Ag/AgCl), respectively. A linear range in the dependence of the current response upon the concentration of lactic acid was up to 1 mM. Electrode sensitivity and apparent Michaelis constants (K-M') decreased with aging of the yeast. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://lmavb.lvb.lt/LMAVB:ELABAPDB5319243&prefLang=en_USTest |
| الإتاحة: | http://lmavb.lvb.lt/LMAVB:ELABAPDB5319243&prefLang=en_USTest |
| رقم الانضمام: | edsbas.5E654B81 |
| قاعدة البيانات: | BASE |
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