دورية أكاديمية
Semi-quantitative estimation of serum myoglobin by a rapid latex agglutination method: an emergency screening test for acute myocardial infarction.
العنوان: | Semi-quantitative estimation of serum myoglobin by a rapid latex agglutination method: an emergency screening test for acute myocardial infarction. |
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المؤلفون: | Chapelle, Jean-Paul, Heusghem, C. |
المصدر: | Clinica Chimica Acta, 145 (2), 143-50 (1985) |
بيانات النشر: | Elsevier Science |
سنة النشر: | 1985 |
المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
مصطلحات موضوعية: | Acute Disease, Adult, Aspartate Aminotransferases/blood, Creatine Kinase/blood, Emergencies, Female, Hepatitis, Viral, Human/enzymology, Humans, Isoenzymes, L-Lactate Dehydrogenase/blood, Latex Fixation Tests/methods, Male, Myocardial Infarction/diagnosis/enzymology, Myoglobin/blood, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale |
الوصف: | peer reviewed ; The present study reports the evaluation of a new latex agglutination test for serum myoglobin (SMb). The time of agglutination of the latex particles coated with antibodies to myoglobin was measured in 172 serum specimens with known concentration of myoglobin quantitated by a radioimmunoassay (RIA), collected from myocardial infarction (MI) patients, subjects suffering from various diseases, and normal controls. Myoglobin levels in the samples were found to decrease exponentially with time of agglutination. Agglutination occurring within 1 min (result coded as + + + +) corresponded to 761 +/- 366 micrograms/l of myoglobin; between 1 and 2 min (+ + +), to 285 +/- 101 micrograms/l; between 2 and 3 min (+ +), to 85 +/- 47 micrograms/l; between 3 and 4 min (+), to 51 +/- 38 micrograms/l; and after more than 4 min (-), to 31 +/- 16 micrograms/l. Blood samples were serially drawn from 24 MI patients with short hospitalization delays; the rapid agglutination which was obtained in the specimens taken upon admission (20 results coded as + + + + and four as + + +) actually corresponded to markedly increased SMb levels. In contrast, serum creatine kinase (CK) activities were still less than 150 U/l in four patients (16.6%); CK-MB was less than 5 U/l in five cases (20.8%). Positive agglutinations for SMb were also obtained 4 and 8 h following admission in all subjects, confirming that the latex test is an early and very sensitive indicator for MI.(ABSTRACT TRUNCATED AT 250 WORDS) |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 0009-8981 1873-3492 |
العلاقة: | urn:issn:0009-8981; urn:issn:1873-3492; https://orbi.uliege.be/handle/2268/34458Test; info:hdl:2268/34458; scopus-id:2-s2.0-0021960221; info:pmid:3971587 |
DOI: | 10.1016/0009-8981(85)90281-5 |
الإتاحة: | https://doi.org/10.1016/0009-8981Test(85)90281-5 https://orbi.uliege.be/handle/2268/34458Test |
رقم الانضمام: | edsbas.3439B6DA |
قاعدة البيانات: | BASE |
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The time of agglutination of the latex particles coated with antibodies to myoglobin was measured in 172 serum specimens with known concentration of myoglobin quantitated by a radioimmunoassay (RIA), collected from myocardial infarction (MI) patients, subjects suffering from various diseases, and normal controls. Myoglobin levels in the samples were found to decrease exponentially with time of agglutination. Agglutination occurring within 1 min (result coded as + + + +) corresponded to 761 +/- 366 micrograms/l of myoglobin; between 1 and 2 min (+ + +), to 285 +/- 101 micrograms/l; between 2 and 3 min (+ +), to 85 +/- 47 micrograms/l; between 3 and 4 min (+), to 51 +/- 38 micrograms/l; and after more than 4 min (-), to 31 +/- 16 micrograms/l. 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