Mesenchymal stem cells suppress lung inflammation and airway remodeling in chronic asthma rat model via PI3K/Akt signaling pathway
العنوان: | Mesenchymal stem cells suppress lung inflammation and airway remodeling in chronic asthma rat model via PI3K/Akt signaling pathway |
---|---|
المؤلفون: | Lin, Hai-Yan, Xu, Lei, Xie, Shuan-Shuan, Yu, Fei, Hu, Hai-Yang, Song, Xiao-Lian, Wang, Chang-Hui |
بيانات النشر: | e-Century Publishing Corporation, 2015. |
سنة النشر: | 2015 |
مصطلحات موضوعية: | Male, Ovalbumin, Pneumonia, respiratory system, Mesenchymal Stem Cell Transplantation, Asthma, respiratory tract diseases, Rats, Rats, Sprague-Dawley, Disease Models, Animal, Phosphatidylinositol 3-Kinases, Airway Remodeling, Animals, Original Article, Bronchoalveolar Lavage Fluid, Lung, Proto-Oncogene Proteins c-akt, Signal Transduction |
الوصف: | Background: Mesenchymal stem cells (MSCs) came out to attract wide attention and had become one of the hotspots of most diseases’ research in decades. But at present, the mechanisms of how MSCs work on chronic asthma remain undefined. Our study aims at verifying whether MSCs play a role in preventing inflammation and airway remodeling via PI3K/AKT signaling pathway in the chronic asthma rats model. Methods: First, an ovalbumin (OVA)-induced asthma model was built. MSCs were administered to ovalbumin-induced asthma rats. The total cells in a bronchial alveolar lavage fluid (BALF) and inflammatory mediators in BALF and serum were measured. Histological examination of lung tissue was performed to estimate the pathological changes. Additionally, the expression of phosphorylated-Akt (p-Akt) in all groups was measured by western blot and immunohistochemistry (IHC). Results: Compared to normal control group, the degree of airway inflammation and airway remodeling was significantly increased in asthma group. On the contrary, they were obviously inhibited in MSCs transplantation group. Moreover, the expression of p-Akt was increased in lung tissues of asthmatic rats, and suppressed by MSCs transplantation. Conclusion: Our results demonstrated that MSCs transplantation could suppress lung inflammation and airway remodeling via PI3K/Akt signaling pathway in rat asthma model. |
اللغة: | English |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::db16e326cf5c5371fb3d9b9008a4af3fTest https://europepmc.org/articles/PMC4583869Test/ |
حقوق: | OPEN |
رقم الانضمام: | edsair.pmid..........db16e326cf5c5371fb3d9b9008a4af3f |
قاعدة البيانات: | OpenAIRE |