[Preventive effects of curcumin on acrylamide-induced DNA damage in HepG2 cells]
| العنوان: | [Preventive effects of curcumin on acrylamide-induced DNA damage in HepG2 cells] |
|---|---|
| المؤلفون: | Jun, Cao, Liping, Jiang, Chengyan, Geng, Xiaofeng, Yao |
| المصدر: | Wei sheng yan jiu = Journal of hygiene research. 38(4) |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Acrylamide, Curcumin, Humans, Comet Assay, Free Radical Scavengers, Hep G2 Cells, Reactive Oxygen Species, DNA Damage |
| الوصف: | To investigate the protective effects of curcumin on acrylamide (AA)-induced DNA damage in HepG2 cells.After pretreatment with curcumin (0.63, 1.25 and 2.50 microg/ml) for 1 hour, HepG2 cells were exposed to various concentrations of AA (0, 10, 20 mmol/L) for 1 h, comet assay was performed to determine DNA damage. The production of reactive oxygen species (ROS) was measured using the 2, 7-dichlorofluorescein diacetate (DCFH-DA) method.One-hour exposure of HepG2 cells to AA led to a dose-dependent increase in DNA fragmentation. When the cells were pretreated with curcumin for 1 h, the comet tail moment values were decreased compared to only AA-treated cells, and curcumin of 2.50 microg/nml significantly decreased the comet tail moment values compared to only AA-treated cells (P0.05). The DCF fluorescence intensity increased when the HepG2 cells were treated with 10 and 20 mmol/L AA (P0.01). When the cells were pretreated with curcumin, the level of ROS was significantly decreased compared to only AA-treated (P0.01).These data suggest that curcumin could attenuate AA-induced DNA damage in HepG2 cells. The protection is probably mediated by antioxidant protective mechanism. |
| تدمد: | 1000-8020 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::1535e50ffa19c36956a295cdc2239ea1Test https://pubmed.ncbi.nlm.nih.gov/19689063Test |
| رقم الانضمام: | edsair.pmid..........1535e50ffa19c36956a295cdc2239ea1 |
| قاعدة البيانات: | OpenAIRE |
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