lncRNA PSMB8-AS1 promotes colorectal cancer progression through sponging miR-1299 to upregulate ADAMTS5
العنوان: | lncRNA PSMB8-AS1 promotes colorectal cancer progression through sponging miR-1299 to upregulate ADAMTS5 |
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المؤلفون: | Fang, Zhao, Meng, Wang, Yibin, Zhang, Rujuan, Su, Chenyang, He, Xiao, Gao, Ying, Zan, Shuqun, Zhang, Yuguang, Ma, Chao, Liu |
المصدر: | Neoplasma. 69:1138-1153 |
بيانات النشر: | AEPress, s.r.o., 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Gene Expression Regulation, Neoplastic, MicroRNAs, Cancer Research, Oncology, Cell Movement, Cell Line, Tumor, Humans, RNA, Long Noncoding, ADAMTS5 Protein, Colorectal Neoplasms, B7-H1 Antigen, Cell Proliferation |
الوصف: | Long non-coding RNAs (lncRNAs) have been reported to be vital participants in tumor progression. Recently, lncRNA PSMB8-AS1 has been uncovered to facilitate pancreatic cancer progression by regulating miR-382-3p/STAT1/PD-L1 network. Nonetheless, the role of PSMB8-AS1 and its underlying mechanism have not been well-explored in colorectal cancer (CRC). The expression of RNAs or proteins was detected via qRT-PCR or western blot assays. Functional assays were involved in evaluating the effects of PSMB8-AS1/miR-1299/ADAMTS5 on the malignant behaviors of CRC cells. The molecular mechanism of PSMB8-AS1 was explored via mechanism analyses in CRC cells. Based on experimental results, PSMB8-AS1 expression was notably higher in CRC cell lines than in normal cells. The downregulation of PSMB8-AS1 repressed cell viability, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC while promoting cell apoptosis. It was also revealed that PSMB8-AS1 could sponge miR-1299 to upregulate ADAMTS5 in CRC cells. In rescue assays, we further discovered that miR-1299 inhibition or ADAMTS5 overexpression abrogated the suppressive influence of PSMB8-AS1 deficiency on CRC cell growth. In addition, PSMB8-AS1 was validated to induce M2 polarization. In conclusion, PSMB8-AS1 sponges miR-1299 to increase PSMB8-AS1 expression, thus promoting CRC cell growth. |
تدمد: | 1338-4317 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d697d1e7d17170ccb431412082845ddcTest https://doi.org/10.4149/neo_2022_220111n42Test |
رقم الانضمام: | edsair.doi.dedup.....d697d1e7d17170ccb431412082845ddc |
قاعدة البيانات: | OpenAIRE |
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