Antioxidant Antagonises Chemotherapeutic Drug Effect in Lung Cancer Cell Line A549
| العنوان: | Antioxidant Antagonises Chemotherapeutic Drug Effect in Lung Cancer Cell Line A549 |
|---|---|
| المؤلفون: | Gnanasambandan Ramanathan, Suresh Kannan Subramanian Shanmugam, Swetha Rajendran, Aishwarya Lakshminarayanan |
| المصدر: | Asian Pacific Journal of Cancer Prevention : APJCP |
| بيانات النشر: | EpiSmart Science Vector Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Drug, Antimetabolites, Antineoplastic, antioxidant, Lung Neoplasms, Antioxidant, media_common.quotation_subject, medicine.medical_treatment, Lung cancer cell line, Apoptosis, Pharmacology, Antioxidants, 03 medical and health sciences, 0302 clinical medicine, A549 cell line, Tumor Cells, Cultured, medicine, Humans, MTT assay, Thioguanine, Lung cancer, chemotherapeutic drug, Cell Proliferation, media_common, A549 cell, Chemistry, Free Radical Scavengers, General Medicine, medicine.disease, Acetylcysteine, 030104 developmental biology, 030220 oncology & carcinogenesis, Drug Therapy, Combination, Chemotherapeutic drugs, Research Article, Cysteine |
| الوصف: | Objective This study aimed to find whether antioxidants increase or decrease the effect of chemotherapeutic drug in the in vitro model. Methods Small lung Cancer cell line (A549) was treated with anticancer drug 6-Thioguanine (6-TG) at different concentration viz., 1, 10, 50 and 100μM and the proliferation was measured using MTT assay. The antioxidant N-Acetyl Cysteine (NAC) in different ratios viz., 1mM, 5mM and 10mM were assayed for their effect in proliferation on the A549 cells alone and in combination with 6-TG. Results Our experiment proves that anticancer drug 6-TG decreases the proliferation and the antioxidant NAC enhances the proliferation of A549 cells. Strikingly when co-treated with 6-TG, the antioxidant NAC diminished the proliferation reduction action of 6-TG on A549 cells. Conclusion Our results suggest that antioxidants in fact benefit the tumor cell growth when treated alone and when in combination with anticancer drug, it severely impair the activity of the drug. We propose that extreme care should be taken when prescribing antioxidants alone or in combination with chemotherapeutics. |
| تدمد: | 2476-762X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d37d712cf308f0a4c7987a9ccf5b6462Test https://doi.org/10.31557/apjcp.2020.21.4.1019Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d37d712cf308f0a4c7987a9ccf5b6462 |
| قاعدة البيانات: | OpenAIRE |
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