GEC1, a protein related to GABARAP, interacts with tubulin and GABA(A) receptor
| العنوان: | GEC1, a protein related to GABARAP, interacts with tubulin and GABA(A) receptor |
|---|---|
| المؤلفون: | Jean Luc Schlick, Annick Fraichard, Michèle Jouvenot, Wilfrid Boireau, Régis Delage-Mourroux, Virginie Mansuy |
| المصدر: | Biochemical and biophysical research communications. 325(2) |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Protein subunit, GABARAP, Recombinant Fusion Proteins, Green Fluorescent Proteins, Molecular Sequence Data, Biophysics, Intracellular Space, Gene Expression, CHO Cells, Biology, Biochemistry, Microtubules, Tubulin, Cricetinae, Escherichia coli, Animals, Humans, Amino Acid Sequence, Receptor, Molecular Biology, Adaptor Proteins, Signal Transducing, Glutathione Transferase, Dose-Response Relationship, Drug, Signal transducing adaptor protein, Cell Biology, ULK1, Receptors, GABA-A, Fusion protein, Cell biology, Protein Structure, Tertiary, Protein Subunits, Cytoplasm, biology.protein, Apoptosis Regulatory Proteins, Microtubule-Associated Proteins, Sequence Alignment, Protein Binding |
| الوصف: | We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA(A) receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA(A) receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA(A) receptor. |
| تدمد: | 0006-291X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d099dbaa535871a58e120e976fd6ab76Test https://pubmed.ncbi.nlm.nih.gov/15530441Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d099dbaa535871a58e120e976fd6ab76 |
| قاعدة البيانات: | OpenAIRE |
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