3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes
| العنوان: | 3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes |
|---|---|
| المؤلفون: | P. B. Gordon, Per Ottar Seglen |
| سنة النشر: | 1982 |
| مصطلحات موضوعية: | Purine, Liver cytology, Protein degradation, Biology, chemistry.chemical_compound, Adenosine Triphosphate, Phagocytosis, Protein biosynthesis, Animals, Amino Acids, Biological Sciences: Cell Biology, Cells, Cultured, chemistry.chemical_classification, Multidisciplinary, Adenine, Autophagy, Proteins, Cell biology, Amino acid, Rats, Biochemistry, chemistry, Liver, Protein Biosynthesis, Autolysis, Lysosomes, Adenosine triphosphate, Intracellular |
| الوصف: | 3-Methyladenine (5 mM) inhibits endogenous protein degradation in isolated rat hepatocytes by about 60%, while having no adverse effect on the degradation of an exogenous protein (asialofetuin), on protein synthesis, or on intracellular ATP levels. 3-Methyladenine appears to act specifically upon the autophagic/lysosomal pathway of degradation, as judged from its lack of effect in the presence of amino acids or a lysosomotropic amine (propylamine). The effect of the purine is not mediated by amino acids because the inhibition of protein degradation is accompanied by a significant depression of intracellular amino acid levels. The ability of 3-methyladenine to suppress the formation of electron microscopically visible autophagosomes suggests that it may be regarded as a specific inhibitor of autophagy. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1babbf577c2810d389d0036c16cd81Test https://europepmc.org/articles/PMC346086Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ce1babbf577c2810d389d0036c16cd81 |
| قاعدة البيانات: | OpenAIRE |
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