Crystallization of mitochondrial cytochrome b-c1 complex from gel with or without reduced pressure
العنوان: | Crystallization of mitochondrial cytochrome b-c1 complex from gel with or without reduced pressure |
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المؤلفون: | Linda Yu, Johann Deisenhofer, Chang An Yu, Di Xia |
المصدر: | Journal of Molecular Biology. 243:802-805 |
بيانات النشر: | Elsevier BV, 1994. |
سنة النشر: | 1994 |
مصطلحات موضوعية: | Cytochrome, Mitochondria, Heart, law.invention, Electron Transport Complex III, Tetragonal crystal system, X-Ray Diffraction, Structural Biology, law, Pressure, Animals, Mother liquor, Crystallization, Molecular Biology, biology, Chemistry, Sepharose, Resolution (electron density), Solvent, Crystallography, Electrophoresis, X-ray crystallography, Solvents, biology.protein, Cattle, Electrophoresis, Polyacrylamide Gel |
الوصف: | Cytochrome b-c1 complex (ubiquinol-cytochrome c reductase) of beef heart mitochondria has been crystallized. Crystals grown in capillary tubes diffracted X-rays from a laboratory source to a resolution of 7 A and synchrotron radiation to a resolution of 4.5 A in the presence of mother liquor. However, the movement of crystals in the mother liquor makes data collection very difficult. Removal of the mother liquor from the crystals causes severe loss of diffraction quality. To circumvent these difficulties we have recently developed a method for crystallization of the cytochrome b-c1 complex from a gel. The sizes, shapes and diffraction qualities of crystals grown in gel approach those of crystals obtained from liquid. Preliminary experiments on a Xuong-Hamlin area detector indicate that these crystals have the symmetry of a body centered tetragonal space group with cell constants a = b = 157 A, c = 590 A. Assuming eight cytochrome b-c1 complex dimers per unit cell, the crystals have a solvent content of 70% (v/v). Under reduced pressure the crystallization time is significantly decreased. Although crystals obtained under reduced pressure are generally smaller, the shorter crystallization time provides an opportunity to explore more crystallization conditions. |
تدمد: | 0022-2836 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca8f3611b042402188a081f846c73af4Test https://doi.org/10.1016/0022-2836Test(94)90051-5 |
حقوق: | CLOSED |
رقم الانضمام: | edsair.doi.dedup.....ca8f3611b042402188a081f846c73af4 |
قاعدة البيانات: | OpenAIRE |