Multi-species microarrays reveal the effect of sequence divergence on gene expression profiles
العنوان: | Multi-species microarrays reveal the effect of sequence divergence on gene expression profiles |
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المؤلفون: | Yoav Gilad, Mark Gerstein, Scott A. Rifkin, Paul Bertone, Kevin P. White |
بيانات النشر: | Cold Spring Harbor Laboratory Press, 2005. |
سنة النشر: | 2005 |
مصطلحات موضوعية: | Male, Primates, Sequence analysis, Computational biology, Biology, Evolution, Molecular, Species Specificity, Complementary DNA, Gene expression, Genetics, Coding region, Animals, Humans, Letters, Genetics (clinical), Sequence (medicine), Oligonucleotide Array Sequence Analysis, Natural selection, Gene Expression Profiling, Computational Biology, Nucleic Acid Hybridization, Sequence Analysis, DNA, Gene expression profiling, Linear Models, DNA microarray |
الوصف: | Interspecies comparisons of gene expression levels will increase our understanding of the evolution of transcriptional mechanisms and help to identify targets of natural selection. This approach holds particular promise for apes, as many human-specific adaptations are thought to result from differences in gene expression rather than in coding sequence. To date, however, all studies directly comparing interspecies gene expression have been performed on single-species arrays, so that it has been impossible to distinguish differential hybridization due to sequence mismatches from underlying expression differences. To evaluate the severity of this potential problem, we constructed a new multiprimate cDNA array using probes from human, chimpanzee, orangutan, and rhesus. We find a large effect of sequence divergence on hybridization signal, even in the closest pair of species, human and chimpanzee. By comparing single-species array analyses with results from multispecies arrays, we examine how estimates of differential gene expression are affected by sequence divergence. Our results indicate that naive use of single-species arrays in direct interspecies comparisons can yield spurious results. |
اللغة: | English |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1a465ad1cadeb7138934e1c74fd48dfTest https://europepmc.org/articles/PMC1088295Test/ |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....c1a465ad1cadeb7138934e1c74fd48df |
قاعدة البيانات: | OpenAIRE |
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