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Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions

التفاصيل البيبلوغرافية
العنوان:	Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions
المؤلفون:	Christian Genin, Karine Passebosc-Faure, Guorong Li, Anne Gentil-Perret, Claude Lambert, Pierre Fournel, Michèle Cottier, Maurice Pérol
المصدر:	Clinical Cancer Research. 11:6862-6867
بيانات النشر:	American Association for Cancer Research (AACR), 2005.
سنة النشر:	2005
مصطلحات موضوعية:	Male, Cancer Research, Pathology, chemistry.chemical_compound, Carcinoembryonic antigen, Cytology, Uteroglobin, MUC1, education.field_of_study, biology, Reverse Transcriptase Polymerase Chain Reaction, Mammaglobin A, Ascites, Epithelial cell adhesion molecule, Middle Aged, Cadherins, Epithelial Cell Adhesion Molecule, Neoplasm Proteins, Serous fluid, Oncology, Calbindin 2, Female, medicine.medical_specialty, Genes, Wilms Tumor, Population, Malignancy, Sensitivity and Specificity, S100 Calcium Binding Protein G, Mammaglobin, Antigens, Neoplasm, Cell Line, Tumor, Biomarkers, Tumor, medicine, Humans, Genetic Predisposition to Disease, Antigens, WT1 Proteins, education, neoplasms, Aged, DNA Primers, Glycoproteins, business.industry, Carcinoma, Mucin-1, Mucins, Oligonucleotides, Antisense, medicine.disease, digestive system diseases, Carcinoembryonic Antigen, Pleural Effusion, Malignant, Pleural Effusion, chemistry, biology.protein, RNA, business, Cell Adhesion Molecules
الوصف:	Purpose: Our main goal was to evaluate a panel of molecular markers for the detection of cancer cells in serous effusions and to determine their value as an adjunctive reverse transcription-PCR (RT-PCR) test to cytologic examination. Experimental Design: One hundred fourteen serous effusions from 71 patients with tumors and 43 patients with benign diseases were subjected to RT-PCR for expression of carcinoembryonic antigen (CEA), epithelial cell adhesion molecule (Ep-CAM), E-cadherin, mammaglobin, mucin 1 (MUC1) isoforms MUC1/REP, MUC1/Y, and MUC1/Z, calretinin, and Wilms' tumor 1 susceptibility gene. Results: CEA, Ep-CAM, E-cadherin, and mammaglobin were specifically expressed in malignant effusions. The sensitivity of RT-PCR in cytologically negative malignant effusions was 63.1% combining CEA and Ep-CAM (with 100% specificity) and reached 78.9% adding MUC1/Y or MUC1/Z (with 93% specificity). In the whole population of effusions, the combination of cytology with RT-PCR of CEA and Ep-CAM yielded a 90.1% sensitivity, a specificity and a positive predictive value of 100%, and a 86% negative predictive value for malignancy. Adding MUC1/Y or MUC1/Z to the panel, the sensitivity was 94.5% with 93% specificity, 95.7% PPV, and 90.9% negative predictive value. Moreover, CEA and mammaglobin were specifically expressed in epithelial malignancies, and mammaglobin was mainly expressed in effusions from breast carcinoma (97.3% of specificity). Conclusions: A combination of cytology and RT-PCR analysis of CEA and Ep-CAM significantly improved the detection sensitivity of tumor cells in serous effusions. RT-PCR analysis of CEA, Ep-CAM, and mammaglobin in serous effusions could be a beneficial adjunct to cytology for the diagnosis of malignancy.
تدمد:	1557-3265 1078-0432
الوصول الحر:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdb483fb389cd6ddcaf9025c8bc31620Test https://doi.org/10.1158/1078-0432.ccr-05-0043Test
حقوق:	OPEN
رقم الانضمام:	edsair.doi.dedup.....bdb483fb389cd6ddcaf9025c8bc31620
قاعدة البيانات:	OpenAIRE

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examination. Experimental Design: One hundred fourteen serous effusions from 71 patients with tumors and 43 patients with benign diseases were subjected to RT-PCR for expression of carcinoembryonic antigen (CEA), epithelial cell adhesion molecule (Ep-CAM), E-cadherin, mammaglobin, mucin 1 (MUC1) isoforms MUC1/REP, MUC1/Y, and MUC1/Z, calretinin, and Wilms' tumor 1 susceptibility gene. Results: CEA, Ep-CAM, E-cadherin, and mammaglobin were specifically expressed in malignant effusions. The sensitivity of RT-PCR in cytologically negative malignant effusions was 63.1% combining CEA and Ep-CAM (with 100% specificity) and reached 78.9% adding MUC1/Y or MUC1/Z (with 93% specificity). In the whole population of effusions, the combination of cytology with RT-PCR of CEA and Ep-CAM yielded a 90.1% sensitivity, a specificity and a positive predictive value of 100%, and a 86% negative predictive value for malignancy. Adding MUC1/Y or MUC1/Z to the panel, the sensitivity was 94.5% with 93% specificity, 95.7% PPV, and 90.9% negative predictive value. Moreover, CEA and mammaglobin were specifically expressed in epithelial malignancies, and mammaglobin was mainly expressed in effusions from breast carcinoma (97.3% of specificity). Conclusions: A combination of cytology and RT-PCR analysis of CEA and Ep-CAM significantly improved the detection sensitivity of tumor cells in serous effusions. 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