Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo
| العنوان: | Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo |
|---|---|
| المؤلفون: | Hong Bae Jeon, Hye Jin Jin, Jae-Sung Kim, Wonil Oh, Mi-Yeon Kim, Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Jueun Ha, Soon-Jae Kwon, Jong Wook Chang, Yoon Sun Yang |
| المصدر: | Biochemical and Biophysical Research Communications. 446:983-989 |
| بيانات النشر: | Elsevier BV, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | T cell, Biophysics, Antigens, CD34, Mice, SCID, Biology, Lymphocyte Activation, Mesenchymal Stem Cell Transplantation, Biochemistry, Interferon-gamma, Mice, Immune system, In vivo, medicine, Animals, Humans, Molecular Biology, Cells, Cultured, Tumor Necrosis Factor-alpha, Immunogenicity, Mesenchymal stem cell, Mesenchymal Stem Cells, Cell Biology, Fetal Blood, Transplantation, Disease Models, Animal, medicine.anatomical_structure, Immunoglobulin G, Humanized mouse, Immunology, Stem cell |
| الوصف: | Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to technical and ethical limitations. Here, we show that allogeneic human umbilical cord blood derived-MSCs (hUCB-MSCs) maintained low immunogenicity even after immune challenge in vitro. To confirm these properties in vivo, a humanized mouse model was established by injecting isolated hUCB-derived CD34+ cells intravenously into immunocompromised NOD/SCID IL2γnull (NSG) mice. After repeated intravenous injection of human peripheral blood mononuclear cells (hPBMCs) or MRC5 cells into these mice, immunological alterations including T cell proliferation and increased IFN-γ, TNF-α, and human IgG levels, were observed. In contrast, hUCB-MSC injection did not elicit these responses. While lymphocyte infiltration in the lung and small intestine and reduced survival rates were observed after hPBMC or MRC5 transplantation, no adverse events were observed following hUCB-MSC introduction. In conclusion, our data suggest that allogeneic hUCB-MSCs have low immunogenicity in vitro and in vivo, and are therefore "immunologically safe" for use in allogeneic clinical applications. |
| تدمد: | 0006-291X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b879efa5520eb6641298a1316fe90a90Test https://doi.org/10.1016/j.bbrc.2014.03.051Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b879efa5520eb6641298a1316fe90a90 |
| قاعدة البيانات: | OpenAIRE |
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