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The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an l-NAME model of pre-eclampsia

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العنوان:	The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an l-NAME model of pre-eclampsia
المؤلفون:	Prem Gathiram, Saravanakumar Murugesan, Irene Mackraj, Sooraj Baijnath, Jagidesa Moodley
المصدر:	Molecular and Cellular Biochemistry. 427:59-67
بيانات النشر:	Springer Science and Business Media LLC, 2016.
سنة النشر:	2016
مصطلحات موضوعية:	0301 basic medicine, medicine.medical_specialty, Clinical chemistry, Urinary system, Clinical Biochemistry, Urine, 030204 cardiovascular system & hematology, Kidney, Peripheral blood mononuclear cell, Sildenafil Citrate, Rats, Sprague-Dawley, Nephrin, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Pre-Eclampsia, Pregnancy, Oral administration, Internal medicine, medicine, Animals, Humans, RNA, Messenger, Molecular Biology, biology, business.industry, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Cell Biology, General Medicine, Rats, Vascular endothelial growth factor, Disease Models, Animal, NG-Nitroarginine Methyl Ester, 030104 developmental biology, Endocrinology, Gene Expression Regulation, chemistry, biology.protein, Podocin, Female, business
الوصف:	We investigated the effects of sildenafil citrate (SC) on podocyturia in N ω-nitro-l-arginine methyl ester hydrochloride (l-NAME) model of pre-eclampsia (PE). One hundred and twenty Sprague–Dawley rats (SDR) were divided into five groups like pregnant control (PC), early-onset PE (EOPE), late-onset PE(LOPE), early and late-onset PE with SC-treated groups [EOPE (SC); LOPE (SC)]. PE was induced in SDR by oral administration of l-NAME in drinking water for 4–8 days for EOPE and 8–14 day for LOPE. The blood pressure, urine volume and total urine protein were increased in EOPE and LOPE groups when compared to PC, and all the above parameters decreased in EOPE (SC) and LOPE (SC) groups when compared to EOPE and LOPE groups, respectively. The EOPE and LOPE groups showed an increase in urinary nephrin mRNA and podocin mRNA levels compared to PC group. Increases in serum and renal soluble fms-like tyrosine kinase-1 (sFlt-1) expression levels and decreases in renal vascular endothelial growth factor (VEGF) expression and serum placenta growth factor (PlGF) levels were observed in EOPE and LOPE groups when compared to PC group. In addition, decreases in serum and renal sFlt-1 expression levels and increases in renal VEGF expression and serum PlGF levels were observed in EOPE (SC) and LOPE (SC) groups when compared to EOPE and LOPE groups, respectively. The light microscopy showed that the renal tissue of l-NAME-treated rats had extensive glomerular damage, tubular damage and infiltration by mononuclear cells when compared to PC group. Therefore, SC ameliorated podocyturia through its effects on the antiangiogenic/angiogenic status in this animal model.
تدمد:	1573-4919 0300-8177
الوصول الحر:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b66527988b59c9e69525254156eeb011Test https://doi.org/10.1007/s11010-016-2897-5Test
حقوق:	CLOSED
رقم الانضمام:	edsair.doi.dedup.....b66527988b59c9e69525254156eeb011
قاعدة البيانات:	OpenAIRE

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One hundred and twenty Sprague–Dawley rats (SDR) were divided into five groups like pregnant control (PC), early-onset PE (EOPE), late-onset PE(LOPE), early and late-onset PE with SC-treated groups [EOPE (SC); LOPE (SC)]. PE was induced in SDR by oral administration of l-NAME in drinking water for 4–8 days for EOPE and 8–14 day for LOPE. The blood pressure, urine volume and total urine protein were increased in EOPE and LOPE groups when compared to PC, and all the above parameters decreased in EOPE (SC) and LOPE (SC) groups when compared to EOPE and LOPE groups, respectively. The EOPE and LOPE groups showed an increase in urinary nephrin mRNA and podocin mRNA levels compared to PC group. Increases in serum and renal soluble fms-like tyrosine kinase-1 (sFlt-1) expression levels and decreases in renal vascular endothelial growth factor (VEGF) expression and serum placenta growth factor (PlGF) levels were observed in EOPE and LOPE groups when compared to PC group. 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