High metabolic rates of 4-hydroxynonenal in brain capillary endothelial cells during hypoxia/reoxygenation
العنوان: | High metabolic rates of 4-hydroxynonenal in brain capillary endothelial cells during hypoxia/reoxygenation |
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المؤلفون: | Steffen Kunstmann, Katharina Mertsch, Tilman Grune, Ingolf E. Blasig |
المصدر: | Europe PubMed Central |
بيانات النشر: | Elsevier BV, 1996. |
سنة النشر: | 1996 |
مصطلحات موضوعية: | medicine.medical_specialty, Capillary endothelial cells, Biology, Blood–brain barrier, 4-Hydroxynonenal, Lipid peroxidation, chemistry.chemical_compound, Internal medicine, medicine, Animals, Endothelium, Hypoxia, Molecular Biology, IC50, Cells, Cultured, Aldehydes, General Neuroscience, Brain, Metabolism, Hypoxia (medical), Capillaries, Oxygen, medicine.anatomical_structure, Endocrinology, chemistry, Biochemistry, Cerebrovascular Circulation, Cattle, Neurology (clinical), Hypoxia reoxygenation, medicine.symptom, Developmental Biology |
الوصف: | We measured the accumulation of 4-hydroxynonenal (HNE), a major lipid peroxidation product during hypoxia/reoxygenation of brain capillary endothelial cells (BCEC). The concentration of HNE after 2 h of hypoxia was 0.23 nmol/mg protein and rose up to 0.28 nmol/mg protein after 30 min of reoxygenation. That reflects a 1.5-fold increase, whereas aortic endothelial cells (AEC) increased the HNE level 5-fold, compared to the control. Therefore, the ability of BCEC to degrade exogenously added HNE was tested. The HNE consumption in BCEC achieved a rate of about 600 nmol · min−1 mg protein−1, about two times higher than in AEC. The higher ability of BCEC to degrade FINE is probably the reason of the 2-fold higher IC50 value against the aldehyde. Therefore, we concluded that the high ability of BCEC to degrade HNE is a substantial part of the secondary antioxidative defense of the brain. |
تدمد: | 0006-8993 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8312ea0c187d1582454cfe91686b0414Test https://doi.org/10.1016/s0006-8993Test(96)01076-1 |
حقوق: | CLOSED |
رقم الانضمام: | edsair.doi.dedup.....8312ea0c187d1582454cfe91686b0414 |
قاعدة البيانات: | OpenAIRE |
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