Production of recombinant enzymes of wide use for research
| العنوان: | Production of recombinant enzymes of wide use for research |
|---|---|
| المؤلفون: | Manzur,María J., Muñoz,Rosana V., Lucero,Adrián A., Juri Ayub,Maximiliano, Alvarez,Sergio E., Ciuffo,Gladys M. |
| المصدر: | Electronic Journal of Biotechnology, Volume: 9, Issue: 3, Published: JUN 2006 Electronic Journal of Biotechnology v.9 n.3 2006 SciELO Chile CONICYT Chile instacron:CONICYT |
| بيانات النشر: | Pontificia Universidad Católica de Valparaíso, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | purification, bioactivity, recombinant enzymes, Applied Microbiology and Biotechnology, protein expression, Biotechnology |
| الوصف: | For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper, we report the preparation of recombinant enzymes, expressed in E. coli strains. The enzymes produced exhibited quite good activity, compared with commercial enzymes, allowing us to replace the last ones for several lab applications. We are reporting changes and modifications to standard protocols described. The standard protocols were modified, i.e. for the purification step of Taq, a temperature dependent procedure was designed. The enzymes produced were used in different applications, such as PCR, RT-PCR, PCR Multiplex and RAPDs molecular markers. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8008731160aab9003b55c597c5eb0559Test http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300023&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8008731160aab9003b55c597c5eb0559 |
| قاعدة البيانات: | OpenAIRE |
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