Measuring Cell Mechanics by Optical Alignment Deformation Spectroscopy
| العنوان: | Measuring Cell Mechanics by Optical Alignment Deformation Spectroscopy |
|---|---|
| المؤلفون: | Keith B. Neeves, David W. M. Marr, Kevin B. Roth |
| المصدر: | Biophysical Journal. (3):177a |
| بيانات النشر: | Biophysical Society. Published by Elsevier Inc. |
| مصطلحات موضوعية: | Materials science, animal structures, Microfluidics, Constitutive equation, Biophysics, Mechanics, macromolecular substances, Deformation (meteorology), Stagnation point, Viscoelasticity, Dashpot, Classical mechanics, Spring (device), Spectroscopy |
| الوصف: | Cell mechanical properties are a useful measure of phenotype that can be quantified by cell deformability. There is a lack of high-throughput methods to investigate the mechanical properties of large populations of individual cells. To address this need, we developed optical alignment deformation spectroscopy (OADS), a technique where hydrodynamic interactions between individual cells are used to create deformation. In OADS, a linear optical trap is used to align two incoming cells in a microfluidic cross-flow geometry, allowing hydrodynamic forces to induce a collision between cells at the stagnation point (see figure). After the interaction, the cells leave the stagnation point and a new pair of cells enters the trap. A convenient model cell to characterize OADS is the human erythrocyte because of its well-known mechanical properties. We fit deformation data of erythrocytes to a linear viscoelastic constitutive model (Voigt). This model incorporates a spring and dashpot in parallel, for the elastic (k) and viscous (η) parameters of the cell, respectively. Our measured values of k = 14.5 μN/m and η = 4.9 μN∗s/m compare favorably with literature values. Our results show OADS has potential as an accurate high-throughput individual cell mechanical cytometer.View Large Image | View Hi-Res Image | Download PowerPoint Slide |
| اللغة: | English |
| تدمد: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2011.11.960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62a71a3a14de58415673f65b772137f5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....62a71a3a14de58415673f65b772137f5 |
| قاعدة البيانات: | OpenAIRE |
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