Depot-Specific Expression of Fibroblast Growth Factors in Human Adipose Tissue
| العنوان: | Depot-Specific Expression of Fibroblast Growth Factors in Human Adipose Tissue |
|---|---|
| المؤلفون: | Björn Carlsson, Yoshiaki Itoh, Lena M. S. Carlsson, Lars Sjöström, Malin Lönn, Margareta Jernås, Jenny M. Johansson, Torsten Olbers, Eva Jennische, Britt G. Gabrielsson, Markku Peltonen, Lars Lönn, Hans Lönroth |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Adult, Fibroblast Growth Factor 9, Male, medicine.medical_specialty, FGF21, Endocrinology, Diabetes and Metabolism, Adipose tissue macrophages, Gene Expression, Medicine (miscellaneous), Adipose tissue, Fibroblast growth factor, Polymerase Chain Reaction, Endocrinology, Internal medicine, Gene expression, Adipocytes, medicine, Humans, Obesity, Receptor, Fibroblast Growth Factor, Type 1, Receptor, Fibroblast Growth Factor, Type 2, Receptor, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Chemistry, Public Health, Environmental and Occupational Health, Nucleic Acid Hybridization, Receptor Protein-Tyrosine Kinases, 3T3-L1, Middle Aged, Immunohistochemistry, Receptors, Fibroblast Growth Factor, Fibroblast Growth Factors, Adipose Tissue, Body Composition, Fibroblast Growth Factor 1, Omentum, Food Science |
| الوصف: | We have investigated the expression of several fibroblast growth factors (FGFs) and FGF-receptors (FGFRs) in human adipose tissue and adipose-tissue cell fractions obtained from both subcutaneous (sc) and omental (om) depots.Adipose tissue (sc and om) was obtained from obese men. Gene expression was analyzed by DNA microarrays in triplicate (n = 6) or by real-time polymerase chain reaction (n = 9).FGF-1, FGF-2, FGF-7, FGF-9, FGF-10, and FGF-18 transcripts were detected in human adipose tissue. The expression of FGF-2, FGF-7, and FGF-10 was similar in sc and om adipose tissue, whereas FGF-1 and FGF-9 were expressed at higher levels in the om adipose tissue. Expression of FGF-18 was only detected in om adipose tissue in two of the subjects. Analysis of cell fractions revealed that FGF-2 was only expressed in adipocytes; FGF-7, FGF-9, and FGF-18 were expressed in the stroma-vascular fraction; and FGF-1 and FGF-10 were expressed in both adipocytes and in the stroma-vascular fraction. FGFR-1 was expressed in both depots in all subjects and in both cell fractions, whereas FGFR-2 expression was undetectable in whole adipose tissue but detectable in the adipocyte fractions from both sc and om depots.We show that several members of the FGF family are expressed in human adipose tissue, and that the expression for some of the FGFs differs between sc and om adipose tissue. Taken together with previously published reports on the biological effects of FGFs on adipose cells, our results suggest that locally expressed FGFs could play role in the regulation of regional adipose tissue mass. |
| تدمد: | 1071-7323 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58f16a0a62aedc62872bffd2078b769eTest https://doi.org/10.1038/oby.2002.83Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....58f16a0a62aedc62872bffd2078b769e |
| قاعدة البيانات: | OpenAIRE |
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