Genotoxic risk and oxidative DNA damage in HepG2 cells exposed to perfluorooctanoic acid
العنوان: | Genotoxic risk and oxidative DNA damage in HepG2 cells exposed to perfluorooctanoic acid |
---|---|
المؤلفون: | Laifu Zhong, Xiaofeng Yao |
المصدر: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 587:38-44 |
بيانات النشر: | Elsevier BV, 2005. |
سنة النشر: | 2005 |
مصطلحات موضوعية: | Carcinoma, Hepatocellular, Health, Toxicology and Mutagenesis, Biology, medicine.disease_cause, Risk Assessment, chemistry.chemical_compound, Dichlorofluorescein, Tumor Cells, Cultured, Genetics, medicine, Humans, Carcinogen, chemistry.chemical_classification, Fluorocarbons, Reactive oxygen species, Micronucleus Tests, Mutagenicity Tests, Liver Neoplasms, Molecular biology, Comet assay, Oxidative Stress, chemistry, Cell culture, Micronucleus test, Perfluorooctanoic acid, Comet Assay, Caprylates, Reactive Oxygen Species, Oxidative stress, DNA Damage |
الوصف: | Perfluorooctanoic acid (C 8 HF 15 O 2 , PFOA) is widely used in various industrial fields for decades and it is environmentally bioaccumulative. PFOA is known as a potent hepatocarcinogen in rodents. But it is not yet clear whether it is also carcinogenic in humans, and the genotoxic effects of PFOA on human cells have not yet been examined. In this study, the genotoxic potential of PFOA was investigated in human hepatoma HepG2 cells in culture using single cell gel electrophoresis (SCGE) assay and micronucleus (MN) assay. In order to clarify the underlying mechanism(s) we measured the intracellular generation of reactive oxygen species (ROS) using dichlorofluorescein diacetate as a fluorochrome. The level of oxidative DNA damage was evaluated by immunocytochemical analysis of 8-hydroxydeoxyguanosine (8-OHdG) in PFOA-treated HepG2 cells. PFOA at 50–400 μM caused DNA strand breaks and at 100–400 μM MN in HepG2 cells both in a dose-dependent manner. Significantly increased levels of ROS and 8-OHdG were observed in these cells. We conclude that PFOA exerts genotoxic effects on HepG2 cells, probably through oxidative DNA damage induced by intracellular ROS. |
تدمد: | 1383-5718 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54a5f447623b1af847f90f8c88fcf516Test https://doi.org/10.1016/j.mrgentox.2005.07.010Test |
حقوق: | CLOSED |
رقم الانضمام: | edsair.doi.dedup.....54a5f447623b1af847f90f8c88fcf516 |
قاعدة البيانات: | OpenAIRE |