أعرض في EDS

Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells

التفاصيل البيبلوغرافية
العنوان:	Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells
المؤلفون:	Mindi He, Chunhai Chen, Yonghui Lu, Min Li, Lingling Yang, Zhou Zhou, Lei Zhang, Zhi-Yu Chen, Chuan Liu, Zhengping Yu
المصدر:	Cellular Physiology and Biochemistry, Vol 39, Iss 3, Pp 961-974 (2016)
بيانات النشر:	S. Karger AG, 2016.
سنة النشر:	2016
مصطلحات موضوعية:	0301 basic medicine, Physiology, Cytotoxicity, medicine.disease_cause, lcsh:Physiology, DNA Glycosylases, Histones, Toxicology, Mice, chemistry.chemical_compound, Bisphenol A, Cadmium Chloride, lcsh:QD415-436, Phosphorylation, OGG1, chemistry.chemical_classification, Cadmium, lcsh:QP1-981, G2 Phase Cell Cycle Checkpoints, Drug Combinations, Comet Assay, Poly(ADP-ribose) Polymerases, hormones, hormone substitutes, and hormone antagonists, endocrine system, Cell Survival, DNA damage, Poly ADP ribose polymerase, chemistry.chemical_element, Air Pollutants, Occupational, Cadmium chloride, lcsh:Biochemistry, 03 medical and health sciences, Phenols, medicine, Animals, Estrogens, Non-Steroidal, Benzhydryl Compounds, Reactive oxygen species, L-Lactate Dehydrogenase, urogenital system, Molecular biology, Comet assay, 030104 developmental biology, Gene Expression Regulation, chemistry, DNA glycosylase, NIH 3T3 Cells, Genotoxicity, Reactive Oxygen Species, DNA Damage
الوصف:	Background: Both cadmium (Cd) and bisphenol A (BPA) are commonly encountered in humans' daily activities, but their combined genotoxic effects remain unclear. Methods: In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehydrogenase (LDH) release. Reactive oxygen species (ROS) production was measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA). And DNA damage was measured by 8-hydroxydeoxyguanosine (8-OHdG), phosphorylated H2AX (γH2AX) and the comet assay. The flow cytometry was used to detect cell cycle distribution, and apoptosis was determined by TUNEL assay and western blot against poly-ADP-ribose polymerase (PARP). Results: The results showed that Cd or BPA treatments alone (with the exception of BPA exposure at 50 μM) did not alter cell viability. However, pre-treatment with Cd aggravated the BPA-induced reduction in cell viability; increased BPA-induced LDH release, ROS production, DNA damage and G2 phase arrest; and elevated BPA-induced TUNEL-positive cells and the expression levels of cleaved PARP. Cd exposure concurrently decreased the expression of 8-oxoguanine-DNA glycosylase-1 (OGG1), whereas OGG1 over-expression abolished the enhancement of Cd on BPA-induced genotoxicity and cytotoxicity. Conclusion: These findings indicate that Cd exposure aggravates BPA-induced genotoxicity and cytotoxicity through OGG1 inhibition.
تدمد:	1421-9778 1015-8987
الوصول الحر:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::544ee9bb44708eb338f3ea755c3033c2Test https://doi.org/10.1159/000447804Test
حقوق:	OPEN
رقم الانضمام:	edsair.doi.dedup.....544ee9bb44708eb338f3ea755c3033c2
قاعدة البيانات:	OpenAIRE

ResultId	1
Header	edsair OpenAIRE edsair.doi.dedup.....544ee9bb44708eb338f3ea755c3033c2 822 3 unknown 821.776672363281
PLink	https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....544ee9bb44708eb338f3ea755c3033c2&custid=s6537998&authtype=sso
FullText	Array ( [Availability] => 0 )
Items	Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells ) Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Mindi+He%22">Mindi He</searchLink><br /><searchLink fieldCode="AR" term="%22Chunhai+Chen%22">Chunhai Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Yonghui+Lu%22">Yonghui Lu</searchLink><br /><searchLink fieldCode="AR" term="%22Min+Li%22">Min Li</searchLink><br /><searchLink fieldCode="AR" term="%22Lingling+Yang%22">Lingling Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou+Zhou%22">Zhou Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Lei+Zhang%22">Lei Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Zhi-Yu+Chen%22">Zhi-Yu Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Chuan+Liu%22">Chuan Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Zhengping+Yu%22">Zhengping Yu</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Cellular Physiology and Biochemistry, Vol 39, Iss 3, Pp 961-974 (2016) ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => S. Karger AG, 2016. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2016 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%220301+basic+medicine%22">0301 basic medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Cytotoxicity%22">Cytotoxicity</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Edisease%5Fcause%22">medicine.disease_cause</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3APhysiology%22">lcsh:Physiology</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+Glycosylases%22">DNA Glycosylases</searchLink><br /><searchLink fieldCode="DE" term="%22Histones%22">Histones</searchLink><br /><searchLink fieldCode="DE" term="%22Toxicology%22">Toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%2Echemical%5Fcompound%22">chemistry.chemical_compound</searchLink><br /><searchLink fieldCode="DE" term="%22Bisphenol+A%22">Bisphenol A</searchLink><br /><searchLink fieldCode="DE" term="%22Cadmium+Chloride%22">Cadmium Chloride</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3AQD415-436%22">lcsh:QD415-436</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphorylation%22">Phosphorylation</searchLink><br /><searchLink fieldCode="DE" term="%22OGG1%22">OGG1</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%2Echemical%5Fclassification%22">chemistry.chemical_classification</searchLink><br /><searchLink fieldCode="DE" term="%22Cadmium%22">Cadmium</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3AQP1-981%22">lcsh:QP1-981</searchLink><br /><searchLink fieldCode="DE" term="%22G2+Phase+Cell+Cycle+Checkpoints%22">G2 Phase Cell Cycle Checkpoints</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+Combinations%22">Drug Combinations</searchLink><br /><searchLink fieldCode="DE" term="%22Comet+Assay%22">Comet Assay</searchLink><br /><searchLink fieldCode="DE" term="%22Poly%28ADP-ribose%29+Polymerases%22">Poly(ADP-ribose) Polymerases</searchLink><br /><searchLink fieldCode="DE" term="%22hormones%2C+hormone+substitutes%2C+and+hormone+antagonists%22">hormones, hormone substitutes, and hormone antagonists</searchLink><br /><searchLink fieldCode="DE" term="%22endocrine+system%22">endocrine system</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Survival%22">Cell Survival</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+damage%22">DNA damage</searchLink><br /><searchLink fieldCode="DE" term="%22Poly+ADP+ribose+polymerase%22">Poly ADP ribose polymerase</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%2Echemical%5Felement%22">chemistry.chemical_element</searchLink><br /><searchLink fieldCode="DE" term="%22Air+Pollutants%2C+Occupational%22">Air Pollutants, Occupational</searchLink><br /><searchLink fieldCode="DE" term="%22Cadmium+chloride%22">Cadmium chloride</searchLink><br /><searchLink fieldCode="DE" term="%22lcsh%3ABiochemistry%22">lcsh:Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%2203+medical+and+health+sciences%22">03 medical and health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Phenols%22">Phenols</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%22">medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Estrogens%2C+Non-Steroidal%22">Estrogens, Non-Steroidal</searchLink><br /><searchLink fieldCode="DE" term="%22Benzhydryl+Compounds%22">Benzhydryl Compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22L-Lactate+Dehydrogenase%22">L-Lactate Dehydrogenase</searchLink><br /><searchLink fieldCode="DE" term="%22urogenital+system%22">urogenital system</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+biology%22">Molecular biology</searchLink><br /><searchLink fieldCode="DE" term="%22Comet+assay%22">Comet assay</searchLink><br /><searchLink fieldCode="DE" term="%22030104+developmental+biology%22">030104 developmental biology</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Expression+Regulation%22">Gene Expression Regulation</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%22">chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+glycosylase%22">DNA glycosylase</searchLink><br /><searchLink fieldCode="DE" term="%22NIH+3T3+Cells%22">NIH 3T3 Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Genotoxicity%22">Genotoxicity</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+Oxygen+Species%22">Reactive Oxygen Species</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+Damage%22">DNA Damage</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Background: Both cadmium (Cd) and bisphenol A (BPA) are commonly encountered in humans' daily activities, but their combined genotoxic effects remain unclear. Methods: In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehydrogenase (LDH) release. Reactive oxygen species (ROS) production was measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA). And DNA damage was measured by 8-hydroxydeoxyguanosine (8-OHdG), phosphorylated H2AX (γH2AX) and the comet assay. The flow cytometry was used to detect cell cycle distribution, and apoptosis was determined by TUNEL assay and western blot against poly-ADP-ribose polymerase (PARP). Results: The results showed that Cd or BPA treatments alone (with the exception of BPA exposure at 50 μM) did not alter cell viability. However, pre-treatment with Cd aggravated the BPA-induced reduction in cell viability; increased BPA-induced LDH release, ROS production, DNA damage and G2 phase arrest; and elevated BPA-induced TUNEL-positive cells and the expression levels of cleaved PARP. Cd exposure concurrently decreased the expression of 8-oxoguanine-DNA glycosylase-1 (OGG1), whereas OGG1 over-expression abolished the enhancement of Cd on BPA-induced genotoxicity and cytotoxicity. Conclusion: These findings indicate that Cd exposure aggravates BPA-induced genotoxicity and cytotoxicity through OGG1 inhibition. ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 1421-9778<br />1015-8987 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::544ee9bb44708eb338f3ea755c3033c2" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::544ee9bb44708eb338f3ea755c3033c2</link><br /><link linkTarget="URL" linkTerm="https://doi.org/10.1159/000447804" linkWindow="_blank">https://doi.org/10.1159/000447804</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....544ee9bb44708eb338f3ea755c3033c2 )
RecordInfo	Array ( [BibEntity] => Array ( [Languages] => Array ( [0] => Array ( [Text] => Undetermined ) ) [PhysicalDescription] => Array ( [Pagination] => Array ( [PageCount] => 14 [StartPage] => 961 ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => 0301 basic medicine [Type] => general ) [1] => Array ( [SubjectFull] => Physiology [Type] => general ) [2] => Array ( [SubjectFull] => Cytotoxicity [Type] => general ) [3] => Array ( [SubjectFull] => medicine.disease_cause [Type] => general ) [4] => Array ( [SubjectFull] => lcsh:Physiology [Type] => general ) [5] => Array ( [SubjectFull] => DNA Glycosylases [Type] => general ) [6] => Array ( [SubjectFull] => Histones [Type] => general ) [7] => Array ( [SubjectFull] => Toxicology [Type] => general ) [8] => Array ( [SubjectFull] => Mice [Type] => general ) [9] => Array ( [SubjectFull] => chemistry.chemical_compound [Type] => general ) [10] => Array ( [SubjectFull] => Bisphenol A [Type] => general ) [11] => Array ( [SubjectFull] => Cadmium Chloride [Type] => general ) [12] => Array ( [SubjectFull] => lcsh:QD415-436 [Type] => general ) [13] => Array ( [SubjectFull] => Phosphorylation [Type] => general ) [14] => Array ( [SubjectFull] => OGG1 [Type] => general ) [15] => Array ( [SubjectFull] => chemistry.chemical_classification [Type] => general ) [16] => Array ( [SubjectFull] => Cadmium [Type] => general ) [17] => Array ( [SubjectFull] => lcsh:QP1-981 [Type] => general ) [18] => Array ( [SubjectFull] => G2 Phase Cell Cycle Checkpoints [Type] => general ) [19] => Array ( [SubjectFull] => Drug Combinations [Type] => general ) [20] => Array ( [SubjectFull] => Comet Assay [Type] => general ) [21] => Array ( [SubjectFull] => Poly(ADP-ribose) Polymerases [Type] => general ) [22] => Array ( [SubjectFull] => hormones, hormone substitutes, and hormone antagonists [Type] => general ) [23] => Array ( [SubjectFull] => endocrine system [Type] => general ) [24] => Array ( [SubjectFull] => Cell Survival [Type] => general ) [25] => Array ( [SubjectFull] => DNA damage [Type] => general ) [26] => Array ( [SubjectFull] => Poly ADP ribose polymerase [Type] => general ) [27] => Array ( [SubjectFull] => chemistry.chemical_element [Type] => general ) [28] => Array ( [SubjectFull] => Air Pollutants, Occupational [Type] => general ) [29] => Array ( [SubjectFull] => Cadmium chloride [Type] => general ) [30] => Array ( [SubjectFull] => lcsh:Biochemistry [Type] => general ) [31] => Array ( [SubjectFull] => 03 medical and health sciences [Type] => general ) [32] => Array ( [SubjectFull] => Phenols [Type] => general ) [33] => Array ( [SubjectFull] => medicine [Type] => general ) [34] => Array ( [SubjectFull] => Animals [Type] => general ) [35] => Array ( [SubjectFull] => Estrogens, Non-Steroidal [Type] => general ) [36] => Array ( [SubjectFull] => Benzhydryl Compounds [Type] => general ) [37] => Array ( [SubjectFull] => Reactive oxygen species [Type] => general ) [38] => Array ( [SubjectFull] => L-Lactate Dehydrogenase [Type] => general ) [39] => Array ( [SubjectFull] => urogenital system [Type] => general ) [40] => Array ( [SubjectFull] => Molecular biology [Type] => general ) [41] => Array ( [SubjectFull] => Comet assay [Type] => general ) [42] => Array ( [SubjectFull] => 030104 developmental biology [Type] => general ) [43] => Array ( [SubjectFull] => Gene Expression Regulation [Type] => general ) [44] => Array ( [SubjectFull] => chemistry [Type] => general ) [45] => Array ( [SubjectFull] => DNA glycosylase [Type] => general ) [46] => Array ( [SubjectFull] => NIH 3T3 Cells [Type] => general ) [47] => Array ( [SubjectFull] => Genotoxicity [Type] => general ) [48] => Array ( [SubjectFull] => Reactive Oxygen Species [Type] => general ) [49] => Array ( [SubjectFull] => DNA Damage [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Mindi He ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Chunhai Chen ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Yonghui Lu ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Min Li ) ) ) [4] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Lingling Yang ) ) ) [5] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Zhou Zhou ) ) ) [6] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Lei Zhang ) ) ) [7] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Zhi-Yu Chen ) ) ) [8] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Chuan Liu ) ) ) [9] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Zhengping Yu ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 01 [Type] => published [Y] => 2016 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-print [Value] => 14219778 ) [1] => Array ( [Type] => issn-print [Value] => 10158987 ) [2] => Array ( [Type] => issn-locals [Value] => edsair ) [3] => Array ( [Type] => issn-locals [Value] => edsairFT ) ) [Numbering] => Array ( [0] => Array ( [Type] => volume [Value] => 39 ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Cellular Physiology and Biochemistry [Type] => main ) ) ) ) ) ) )
IllustrationInfo