Intranasal immunization with Mycobacterium tuberculosis Rv3615c induces sustained adaptive CD4+ T-cell and antibody responses in the respiratory tract
| العنوان: | Intranasal immunization with Mycobacterium tuberculosis Rv3615c induces sustained adaptive CD4+ T-cell and antibody responses in the respiratory tract |
|---|---|
| المؤلفون: | Changyou Wu, Jun Zhao, Jiangping Li, Jie Liu, Juan Shen |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, 0301 basic medicine, Tuberculosis, medicine.medical_treatment, mucosal, Epitope, Th1, Mycobacterium tuberculosis, Mice, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Animals, Medicine, Lung, Tuberculosis, Pulmonary, Administration, Intranasal, Antigens, Bacterial, biology, business.industry, intranasal, Vaccination, Original Articles, adaptive immunity, Cell Biology, biology.organism_classification, Acquired immune system, medicine.disease, CD4, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Immunization, 030220 oncology & carcinogenesis, Antibody Formation, Immunology, Cytokines, Molecular Medicine, Original Article, Female, Rv3615c, business, Immunologic Memory, Respiratory tract |
| الوصف: | Sustained adaptive immunity to pathogens provides effective protection against infections, and effector cells located at the site of infection ensure rapid response to the challenge. Both are essential for the success of vaccine development. To explore new vaccination approach against Mycobacterium tuberculosis (M.tb) infection, we have shown that Rv3615c, identified as ESX‐1 substrate protein C of M.tb but not expressed in BCG, induced a dominant Th1‐type response of CD4+ T cells from patients with tuberculosis pleurisy, which suggests a potential candidate for vaccine development. But subcutaneous immunization with Rv3615c induced modest T‐cell responses systemically, and showed suboptimal protection against virulent M.tb challenge at the site of infection. Here, we use a mouse model to demonstrate that intranasal immunization with Rv3615c induces sustained capability of adaptive CD4+ T‐ and B‐cell responses in lung parenchyma and airway. Rv3615c contains a dominant epitope of mouse CD4+ T cells, Rv3615c41‐50, and elicits CD4+ T‐cell response with an effector–memory phenotype and multi‐Th1‐type cytokine coexpressions. Since T cells resident at mucosal tissue are potent at control of infection at early stage, our data show that intranasal immunization with Rv3615c promotes a sustained regional immunity to M.tb, and suggests a potency in control of M.tb infection. Our study warranties a further investigation of Rv3615c as a candidate for development of effective vaccination against M.tb infection. |
| تدمد: | 1582-1838 |
| DOI: | 10.1111/jcmm.13965 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::491ed37dc1ba1ecef5588c1745163b44Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....491ed37dc1ba1ecef5588c1745163b44 |
| قاعدة البيانات: | OpenAIRE |
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Both are essential for the success of vaccine development. To explore new vaccination approach against Mycobacterium tuberculosis (M.tb) infection, we have shown that Rv3615c, identified as ESX‐1 substrate protein C of M.tb but not expressed in BCG, induced a dominant Th1‐type response of CD4+ T cells from patients with tuberculosis pleurisy, which suggests a potential candidate for vaccine development. But subcutaneous immunization with Rv3615c induced modest T‐cell responses systemically, and showed suboptimal protection against virulent M.tb challenge at the site of infection. Here, we use a mouse model to demonstrate that intranasal immunization with Rv3615c induces sustained capability of adaptive CD4+ T‐ and B‐cell responses in lung parenchyma and airway. Rv3615c contains a dominant epitope of mouse CD4+ T cells, Rv3615c41‐50, and elicits CD4+ T‐cell response with an effector–memory phenotype and multi‐Th1‐type cytokine coexpressions. Since T cells resident at mucosal tissue are potent at control of infection at early stage, our data show that intranasal immunization with Rv3615c promotes a sustained regional immunity to M.tb, and suggests a potency in control of M.tb infection. 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