Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveri▿ †
العنوان: | Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveri▿ † |
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المؤلفون: | Fu-Li Li, Julia Hinderberger, Henning Seedorf, Jin Zhang, Wolfgang Buckel, Rudolf K. Thauer |
بيانات النشر: | American Society for Microbiology (ASM), 2007. |
سنة النشر: | 2007 |
مصطلحات موضوعية: | chemistry.chemical_classification, Butyryl-CoA Dehydrogenase, biology, Clostridium kluyveri, Hydrogenosome, Physiology and Metabolism, Dehydrogenase, biology.organism_classification, NAD, Microbiology, Electron-transferring flavoprotein, Glycerol-3-phosphate dehydrogenase, Biochemistry, chemistry, Oxidoreductase, biology.protein, Ferredoxins, lipids (amino acids, peptides, and proteins), NAD+ kinase, Acyl Coenzyme A, Molecular Biology, Ferredoxin |
الوصف: | Cell extracts of butyrate-forming clostridia have been shown to catalyze acetyl-coenzyme A (acetyl-CoA)- and ferredoxin-dependent formation of H 2 from NADH. It has been proposed that these bacteria contain an NADH:ferredoxin oxidoreductase which is allosterically regulated by acetyl-CoA. We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously observed is due to the fact that the cell extracts catalyze the reduction of acetyl-CoA with NADH via crotonyl-CoA to butyryl-CoA. The cytoplasmic butyryl-CoA dehydrogenase complex was purified and is shown to couple the endergonic reduction of ferredoxin (E 0 ′ = −410 mV) with NADH (E 0 ′ = −320 mV) to the exergonic reduction of crotonyl-CoA to butyryl-CoA (E 0 ′ = −10 mV) with NADH. The stoichiometry of the fully coupled reaction is extrapolated to be as follows: 2 NADH + 1 oxidized ferredoxin + 1 crotonyl-CoA = 2 NAD + + 1 ferredoxin reduced by two electrons + 1 butyryl-CoA. The implications of this finding for the energy metabolism of butyrate-forming anaerobes are discussed in the accompanying paper. |
اللغة: | English |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b194580d753a92c905916ee92abfbb0Test https://europepmc.org/articles/PMC2223550Test/ |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....3b194580d753a92c905916ee92abfbb0 |
قاعدة البيانات: | OpenAIRE |
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