Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis
| العنوان: | Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis |
|---|---|
| المؤلفون: | Christine Segonds, Ludovic Lemee, Caroline Thomas, Patrick Honderlick, Marie-Odile Husson, Mohamed Zebouh, Frédéric Wallet |
| المصدر: | Journal of Cystic Fibrosis. 7:238-243 |
| بيانات النشر: | Elsevier BV, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Adult, Male, Pulmonary and Respiratory Medicine, Imipenem, Adolescent, Ceftazidime, Microbial Sensitivity Tests, Aztreonam, medicine.disease_cause, Cystic fibrosis, Microbiology, chemistry.chemical_compound, Tobramycin, medicine, Humans, E test, Pediatrics, Perinatology, and Child Health, business.industry, Pseudomonas aeruginosa, Sputum, Antimicrobial, chemistry, Ticarcillin, Pediatrics, Perinatology and Child Health, Female, Overall MIC, medicine.symptom, business, medicine.drug |
| الوصف: | Background Chronic Pseudomonas aeruginosa colonisation and subsequent exacerbations in patients with cystic fibrosis (CF) require antimicrobial treatment. But since multiple morphotypes and other Gram-negative bacteria with different antibiotic susceptibilities are often isolated inside the same sputum sample, bacteriological analysis is difficult. Methods To simplify this analysis, we explored a direct sputum antimicrobial susceptibility testing (DSST) method by applying E test directly on plates inoculated with the sputum. A total of 316 samples collected from CF patients were analysed and compared with standard procedures (SP) for the identification and antimicrobial susceptibility testing of all Gram-negative bacterial species. Results DSST was as efficient as SP to detect P. aeruginosa including the mucoid morphotype in monomicrobial specimen, but was less sensible to detect all Gram-negative bacteria present in the same sample. It allowed the direct reading of the MIC inhibiting all Gram-negative bacteria. Agreements between these global MICs with the cumulative antibiotics susceptibility of all Gram-negative bacteria measured by SP were excellent for tobramycin and imipenem (>96%) and satisfactory for ticarcillin, ceftazidime, aztreonam and ciprofloxacin (90.4% to 94.3%). In conclusion, the DSST method is an efficient and easy antibiotic susceptibility testing method. |
| تدمد: | 1569-1993 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32d110944c0252ac9f65fd2be63b52cbTest https://doi.org/10.1016/j.jcf.2007.10.002Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....32d110944c0252ac9f65fd2be63b52cb |
| قاعدة البيانات: | OpenAIRE |
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