Indirect estimation of the area density of Atg8 on the phagophore
العنوان: | Indirect estimation of the area density of Atg8 on the phagophore |
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المؤلفون: | Daniel J. Klionsky, Zhiping Xie, Edward D. Rothman, Jiefei Geng, Usha Nair, Maciej B. Szefler |
المصدر: | Autophagy. 5(2) |
سنة النشر: | 2008 |
مصطلحات موضوعية: | Autophagosome, Saccharomyces cerevisiae Proteins, Vesicle, ATG8, Cytoplasmic Vesicles, Cell Biology, Autophagy-Related Protein 8 Family, Saccharomyces cerevisiae, Biology, Autophagosome formation, Article, law.invention, Cell biology, Through transmission, law, Phagosomes, Biophysics, Autophagy, Computer Simulation, Area density, Electron microscope, Molecular Biology, COPII, Microtubule-Associated Proteins |
الوصف: | Atg8 is a ubiquitin-like protein that controls the expansion of the phagophore during autophagosome formation. It is recruited to the phagophore during the expansion stage and released upon the completion of the autophagosome. One possible model explaining the function of Atg8 is that it acts as an adaptor of a coat complex. Here, we tested the coat-adaptor model by estimating the area density of Atg8 molecules on the phagophore. We developed a computational process to simulate the random sectioning of vesicles heterogeneous in size. This method can be applied to estimate the original sizes of intracellular vesicles from sizes of their random sections obtained through transmission electron microscopy. Using this method, we found that the estimated area density of Atg8 is comparable with that of proteins that form the COPII coat. |
تدمد: | 1554-8635 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a18e6ab81a703c93a543e22521e89abTest https://pubmed.ncbi.nlm.nih.gov/19088501Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....2a18e6ab81a703c93a543e22521e89ab |
قاعدة البيانات: | OpenAIRE |