GABA(A) receptor chloride channels are involved in the neuroprotective role of GABA following oxygen and glucose deprivation in the rat cerebral cortex but not in the hippocampus
العنوان: | GABA(A) receptor chloride channels are involved in the neuroprotective role of GABA following oxygen and glucose deprivation in the rat cerebral cortex but not in the hippocampus |
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المؤلفون: | Amy V. Poole, Beatriz Martínez-Villayandre, Mark G. Darlison, Diego Pérez-Rodríguez, Arsenio Fernández-López, Severiano Dos-Anjos, Irene L. Llorente |
المصدر: | Brain research. 1533 |
سنة النشر: | 2013 |
مصطلحات موضوعية: | Male, medicine.medical_specialty, Cell Survival, Amino Acid Transport System X-AG, Glutamate decarboxylase, Hippocampus, Biology, In Vitro Techniques, GABAA-rho receptor, Rats, Sprague-Dawley, Glutamatergic, Internal medicine, medicine, Animals, Molecular Biology, gamma-Aminobutyric Acid, Cerebral Cortex, GABAA receptor, General Neuroscience, Bicuculline, Receptors, GABA-A, Cell Hypoxia, Rats, medicine.anatomical_structure, Endocrinology, Glucose, Neuroprotective Agents, nervous system, Biochemistry, Cerebral cortex, Reperfusion, GABAergic, Neurology (clinical), Developmental Biology, medicine.drug |
الوصف: | Assays on "ex vivo" sections of rat hippocampus and rat cerebral cortex, subjected to oxygen and glucose deprivation (OGD) and a three-hour reperfusion-like (RL) recovery, were performed in the presence of either GABA or the GABA(A) receptor binding site antagonist, bicuculline. Lactate dehydrogenase (LDH) and propidium iodide were used to quantify cell mortality. We also measured, using real-time quantitative polymerase chain reaction (qPCR), the early transcriptional response of a number of genes of the glutamatergic and GABAergic systems. Specifically, glial pre- and post-synaptic glutamatergic transporters (namely GLAST1a, EAAC-1, GLT-1 and VGLUT1), three GABAA receptor subunits (α1, β2 and γ2), and the GABAergic presynaptic marker, glutamic acid decarboxylase (GAD65), were studied. Mortality assays revealed that GABAA receptor chloride channels play an important role in the neuroprotective effect of GABA in the cerebral cortex, but have a much smaller effect in the hippocampus. We also found that GABA reverses the OGD-dependent decrease in GABA(A) receptor transcript levels, as well as mRNA levels of the membrane and vesicular glutamate transporter genes. Based on the markers used, we conclude that OGD results in differential responses in the GABAergic presynaptic and postsynaptic systems. |
تدمد: | 1872-6240 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a0c266186e38c78a66016c05a893737Test https://pubmed.ncbi.nlm.nih.gov/23969196Test |
رقم الانضمام: | edsair.doi.dedup.....2a0c266186e38c78a66016c05a893737 |
قاعدة البيانات: | OpenAIRE |