miR-199a-5p inhibits the progression of papillary thyroid carcinoma by targeting SNAI1
| العنوان: | miR-199a-5p inhibits the progression of papillary thyroid carcinoma by targeting SNAI1 |
|---|---|
| المؤلفون: | Sugang Ma, Wei Jia, Shiyu Ni |
| المصدر: | Biochemical and biophysical research communications. 497(1) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, endocrine system diseases, Biophysics, Mice, Nude, Biochemistry, Papillary thyroid cancer, Thyroid carcinoma, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Western blot, medicine, Tumor Cells, Cultured, Animals, Humans, Neoplasm Invasiveness, Epithelial–mesenchymal transition, Thyroid Neoplasms, Molecular Biology, Cell Proliferation, Gene knockdown, Mice, Inbred BALB C, medicine.diagnostic_test, Chemistry, Cell migration, Cell Biology, Middle Aged, medicine.disease, Carcinoma, Papillary, MicroRNAs, 030104 developmental biology, Thyroid Cancer, Papillary, 030220 oncology & carcinogenesis, SNAI1, Cancer research, Female, Snail Family Transcription Factors, Protein Binding |
| الوصف: | Background Increasing evidence has emphasized the important roles of differentially expressed miRNAs in papillary thyroid cancer (PTC) development. miR-199a-5p was previously documented to be downregulated in PTCs compared with normal thyroids. However, the role of miR-199a-5p in the progression of PTC and the underlying mechanism remain to be further addressed. Methods miR-199a-5p and snail family zinc finger 1 (SNAI1) mRNA expressions in PTC tissues and cells were detected by qRT-PCR. The effects of miR-199a-5p and SNAI1 on cell migration, invasion and epithelial-mesenchymal transition (EMT) were evaluated by cell migration and invasion assays, and western blot, respectively. The relationship between miR-199a-5p and SNAI1 was investigated by luciferase reporter assay and western blot. Xenograft tumor assay was performed to verify the role of miR-199a-5p and molecular mechanism in PTC. Results miR-199a-5p expression was significantly downregulated and SNAI1 was markedly upregulated in PTC tissues and cells. miR-199a-5p overexpression and SNAI1 knockdown suppressed the progression of PTC cells in vitro, as evidenced by the reduced cell migration, invasion and EMT. Of note, SNAI1 was identified as a target of miR-199a-5p and miR-199a-5p suppressed SNAI1 expression in PTC cells. Xenograft tumor assay proved that miR-199a-5p overexpression suppressed tumor growth in PTC in vivo by downregulating SNAI1. Conclusion miR-199a-5p inhibited the progression of PTC by downregulating SNAI1, offering new insight into the molecular mechanism underlying PTC progression. |
| تدمد: | 1090-2104 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::276405bb554e27a5e9cc4f1eb7d3b4b4Test https://pubmed.ncbi.nlm.nih.gov/29427661Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....276405bb554e27a5e9cc4f1eb7d3b4b4 |
| قاعدة البيانات: | OpenAIRE |
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Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Sugang+Ma%22">Sugang Ma</searchLink><br /><searchLink fieldCode="AR" term="%22Wei+Jia%22">Wei Jia</searchLink><br /><searchLink fieldCode="AR" term="%22Shiyu+Ni%22">Shiyu Ni</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <i>Biochemical and biophysical research communications</i>. 497(1) ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2018 ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%220301+basic+medicine%22">0301 basic medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Adult%22">Adult</searchLink><br /><searchLink fieldCode="DE" term="%22Male%22">Male</searchLink><br /><searchLink fieldCode="DE" term="%22endocrine+system+diseases%22">endocrine system diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Biophysics%22">Biophysics</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%2C+Nude%22">Mice, Nude</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Papillary+thyroid+cancer%22">Papillary thyroid cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Thyroid+carcinoma%22">Thyroid carcinoma</searchLink><br /><searchLink fieldCode="DE" term="%2203+medical+and+health+sciences%22">03 medical and health sciences</searchLink><br /><searchLink fieldCode="DE" term="%220302+clinical+medicine%22">0302 clinical medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Downregulation+and+upregulation%22">Downregulation and upregulation</searchLink><br /><searchLink fieldCode="DE" term="%22Western+blot%22">Western blot</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%22">medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor+Cells%2C+Cultured%22">Tumor Cells, Cultured</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Neoplasm+Invasiveness%22">Neoplasm Invasiveness</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial–mesenchymal+transition%22">Epithelial–mesenchymal transition</searchLink><br /><searchLink fieldCode="DE" term="%22Thyroid+Neoplasms%22">Thyroid Neoplasms</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+Biology%22">Molecular Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Proliferation%22">Cell Proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+knockdown%22">Gene knockdown</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%2C+Inbred+BALB+C%22">Mice, Inbred BALB C</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Ediagnostic%5Ftest%22">medicine.diagnostic_test</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+migration%22">Cell migration</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Biology%22">Cell Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Middle+Aged%22">Middle Aged</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Edisease%22">medicine.disease</searchLink><br /><searchLink fieldCode="DE" term="%22Carcinoma%2C+Papillary%22">Carcinoma, Papillary</searchLink><br /><searchLink fieldCode="DE" term="%22MicroRNAs%22">MicroRNAs</searchLink><br /><searchLink fieldCode="DE" term="%22030104+developmental+biology%22">030104 developmental biology</searchLink><br /><searchLink fieldCode="DE" term="%22Thyroid+Cancer%2C+Papillary%22">Thyroid Cancer, Papillary</searchLink><br /><searchLink fieldCode="DE" term="%22030220+oncology+%26+carcinogenesis%22">030220 oncology & carcinogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22SNAI1%22">SNAI1</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+research%22">Cancer research</searchLink><br /><searchLink fieldCode="DE" term="%22Female%22">Female</searchLink><br /><searchLink fieldCode="DE" term="%22Snail+Family+Transcription+Factors%22">Snail Family Transcription Factors</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+Binding%22">Protein Binding</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Background Increasing evidence has emphasized the important roles of differentially expressed miRNAs in papillary thyroid cancer (PTC) development. miR-199a-5p was previously documented to be downregulated in PTCs compared with normal thyroids. However, the role of miR-199a-5p in the progression of PTC and the underlying mechanism remain to be further addressed. Methods miR-199a-5p and snail family zinc finger 1 (SNAI1) mRNA expressions in PTC tissues and cells were detected by qRT-PCR. The effects of miR-199a-5p and SNAI1 on cell migration, invasion and epithelial-mesenchymal transition (EMT) were evaluated by cell migration and invasion assays, and western blot, respectively. The relationship between miR-199a-5p and SNAI1 was investigated by luciferase reporter assay and western blot. Xenograft tumor assay was performed to verify the role of miR-199a-5p and molecular mechanism in PTC. Results miR-199a-5p expression was significantly downregulated and SNAI1 was markedly upregulated in PTC tissues and cells. miR-199a-5p overexpression and SNAI1 knockdown suppressed the progression of PTC cells in vitro, as evidenced by the reduced cell migration, invasion and EMT. Of note, SNAI1 was identified as a target of miR-199a-5p and miR-199a-5p suppressed SNAI1 expression in PTC cells. Xenograft tumor assay proved that miR-199a-5p overexpression suppressed tumor growth in PTC in vivo by downregulating SNAI1. Conclusion miR-199a-5p inhibited the progression of PTC by downregulating SNAI1, offering new insight into the molecular mechanism underlying PTC progression. ) Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 1090-2104 ) Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::276405bb554e27a5e9cc4f1eb7d3b4b4" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::276405bb554e27a5e9cc4f1eb7d3b4b4</link><br /><link linkTarget="URL" linkTerm="https://pubmed.ncbi.nlm.nih.gov/29427661" linkWindow="_blank">https://pubmed.ncbi.nlm.nih.gov/29427661</link> ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => CLOSED ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....276405bb554e27a5e9cc4f1eb7d3b4b4 ) |
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