Multiparametric imaging reveals that mitochondria-rich intercalated cells in the kidney collecting duct have a very high glycolytic capacity
| العنوان: | Multiparametric imaging reveals that mitochondria-rich intercalated cells in the kidney collecting duct have a very high glycolytic capacity |
|---|---|
| المؤلفون: | Robert J. Unwin, Joana Raquel Martins, Soline Bourgeois, Dominik Haenni, Susan Ghazi, César Nombela-Arrieta, Carsten A. Wagner, Alvaro Gomariz, Andrew M. Hall, Milica Bugarski, Eilidh Craigie |
| المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(6) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Mitochondrion, Biochemistry, 03 medical and health sciences, Mice, 0302 clinical medicine, Live cell imaging, Genetics, medicine, Animals, Homeostasis, Intercalated Cell, Glycolysis, Kidney Tubules, Collecting, Molecular Biology, Adenosine Triphosphatases, Epididymis, Kidney, ATP synthase, biology, Chemistry, Epithelial Cells, Metabolism, Hydrogen-Ion Concentration, Proton Pumps, Cell biology, Mitochondria, Mice, Inbred C57BL, Proton-Translocating ATPases, 030104 developmental biology, medicine.anatomical_structure, biology.protein, Calcium, NAD+ kinase, 030217 neurology & neurosurgery, Biotechnology |
| الوصف: | Alpha intercalated cells (αICs) in the kidney collecting duct (CD) belong to a family of mitochondria rich cells (MRCs) and have a crucial role in acidifying the urine via apical V-ATPase pumps. The nature of metabolism in αICs and its relationship to transport was not well-understood. Here, using multiphoton live cell imaging in mouse kidney tissue, FIB-SEM, and other complementary techniques, we provide new insights into mitochondrial structure and function in αICs. We show that αIC mitochondria have a rounded structure and are not located in close proximity to V-ATPase containing vesicles. They display a bright NAD(P)H fluorescence signal and low uptake of voltage-dependent dyes, but are energized by a pH gradient. However, expression of complex V (ATP synthase) is relatively low in αICs, even when stimulated by metabolic acidosis. In contrast, anaerobic glycolytic capacity is surprisingly high, and sufficient to maintain intracellular calcium homeostasis in the presence of complete aerobic inhibition. Moreover, glycolysis is essential for V-ATPase-mediated proton pumping. Key findings were replicated in narrow/clear cells in the epididymis, also part of the MRC family. In summary, using a range of cutting-edge techniques to investigate αIC metabolism in situ, we have discovered that these mitochondria dense cells have a high glycolytic capacity. |
| تدمد: | 1530-6860 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23645158c713c3781010a204cbec6613Test https://pubmed.ncbi.nlm.nih.gov/32367531Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....23645158c713c3781010a204cbec6613 |
| قاعدة البيانات: | OpenAIRE |
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