PER-1- and OXA-10-like beta-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey
العنوان: | PER-1- and OXA-10-like beta-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey |
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المؤلفون: | Zerrin Aktaş, Laurent Poirel, Melek Salcioglu, P. Nordmann, Özdem Anğ, P E Ozcan, Çiğdem Bal, Kenan Midilli |
المصدر: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 11(3) |
سنة النشر: | 2005 |
مصطلحات موضوعية: | Microbiology (medical), OXA-10, Imipenem, Turkey, Cefepime, Ceftazidime, Aztreonam, Biology, medicine.disease_cause, intensive care unit, PER-1, beta-Lactamases, Microbiology, chemistry.chemical_compound, polycyclic compounds, medicine, Humans, Pseudomonas Infections, ceftazidime resistance, β-Lactamase, Antibacterial agent, Cephalosporin Resistance, Pseudomonas aeruginosa, General Medicine, RAPD, Anti-Bacterial Agents, Intensive Care Units, Infectious Diseases, chemistry, Piperacillin, medicine.drug |
الوصف: | The presence of PER-1- and OXA-10-like beta-lactamases was investigated by PCR in 49 ceftazidime-resistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February 2000. The clonal relatedness of the isolates was investigated by random amplified polymorphic DNA (RAPD) analysis, and the sequences of the PER-1 and OXA genes from all isolates were determined. The rates of resistance of the isolates to imipenem, aztreonam and cefepime were 98%, 92% and 96%, respectively, and to piperacillin and piperacillin-tazobactam were 41% and 37%, respectively. Using the double-disk synergy test, 37% (18/49) of the isolates were identified as extended-spectrum beta-lactamase producers. The PER-1 gene was identified in 86% (42/49) and the OXA-10 gene in 55% (27/49) of the ceftazidime-resistant isolates. Of isolates carrying the PER-1 gene, 48% (20/42) also carried the OXA-10 gene. The respective nucleotide sequences were identical for each isolate. Sixteen RAPD patterns were detected among the PER-1-positive isolates, but 60% (25/42) of the PER-1-positive isolates belonged to two distinct patterns, while the remainder exhibited a wide clonal diversity. The results indicated that the prevalence of PER-1- and OXA-10-like beta-lactamases remains high among ceftazidime-resistant P. aeruginosa isolates in Turkey. |
تدمد: | 1198-743X |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0320f7bef5879af61c84bad401105862Test https://pubmed.ncbi.nlm.nih.gov/15715716Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....0320f7bef5879af61c84bad401105862 |
قاعدة البيانات: | OpenAIRE |
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